Reference - Detail
RRC ID | 56230 |
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Author | Ujisawa T, Ohta A, Ii T, Minakuchi Y, Toyoda A, Ii M, Kuhara A. |
Title | Endoribonuclease ENDU-2 regulates multiple traits including cold tolerance via cell autonomous and nonautonomous controls in Caenorhabditis elegans. |
Journal | Proc Natl Acad Sci U S A |
Abstract |
Environmental temperature acclimation is essential to animal survival, yet thermoregulation mechanisms remain poorly understood. We demonstrate cold tolerance in Caenorhabditis elegans as regulated by paired ADL chemosensory neurons via Ca2+-dependent endoribonuclease (EndoU) ENDU-2. Loss of ENDU-2 function results in life span, brood size, and synaptic remodeling abnormalities in addition to enhanced cold tolerance. Enzymatic ENDU-2 defects localized in the ADL and certain muscle cells led to increased cold tolerance in endu-2 mutants. Ca2+ imaging revealed ADL neurons were responsive to temperature stimuli through transient receptor potential (TRP) channels, concluding that ADL function requires ENDU-2 action in both cell-autonomous and cell-nonautonomous mechanisms. ENDU-2 is involved in caspase expression, which is central to cold tolerance and synaptic remodeling in dorsal nerve cord. We therefore conclude that ENDU-2 regulates cell type-dependent, cell-autonomous, and cell-nonautonomous cold tolerance. |
Volume | 115(35) |
Pages | 8823-8828 |
Published | 2018-8-28 |
DOI | 10.1073/pnas.1808634115 |
PII | 1808634115 |
PMID | 30104389 |
PMC | PMC6126727 |
MeSH | Acclimatization / physiology* Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / biosynthesis Caenorhabditis elegans Proteins / genetics Caspases / biosynthesis Caspases / genetics Endoribonucleases / genetics Endoribonucleases / metabolism* Gene Expression Profiling Quantitative Trait, Heritable* Signal Transduction / physiology* Synapses / genetics Synapses / metabolism* TRPC Cation Channels / genetics TRPC Cation Channels / metabolism |
IF | 9.58 |
Times Cited | 7 |
Resource | |
C.elegans | tm4977 tm2644 tm1099 |