Reference - Detail
| RRC ID | 56240 |
|---|---|
| Author | Xu F, Feng X, Chen X, Weng C, Yan Q, Xu T, Hong M, Guang S. |
| Title | A Cytoplasmic Argonaute Protein Promotes the Inheritance of RNAi. |
| Journal | Cell Rep |
| Abstract |
RNAi-elicited gene silencing is heritable and can persist for multiple generations after its initial induction in C. elegans. However, the mechanism by which parental-acquired trait-specific information from RNAi is inherited by the progenies is not fully understood. Here, we identified a cytoplasmic Argonaute protein, WAGO-4, necessary for the inheritance of RNAi. WAGO-4 exhibits asymmetrical translocation to the germline during early embryogenesis, accumulates at the perinuclear foci in the germline, and is required for the inheritance of exogenous RNAi targeting both germline- and soma-expressed genes. WAGO-4 binds to 22G-RNAs and their mRNA targets. Interestingly, WAGO-4-associated endogenous 22G-RNAs target the same cohort of germline genes as CSR-1 and contain untemplated addition of uracil at the 3' ends. The poly(U) polymerase CDE-1 is required for the untemplated uridylation of 22G-RNAs and inheritance of RNAi. Therefore, we conclude that, in addition to the nuclear RNAi pathway, the cytoplasmic RNAi machinery also promotes RNAi inheritance. |
| Volume | 23(8) |
| Pages | 2482-2494 |
| Published | 2018-5-22 |
| DOI | 10.1016/j.celrep.2018.04.072 |
| PII | S2211-1247(18)30631-4 |
| PMID | 29791857 |
| MeSH | Animals Argonaute Proteins / metabolism* Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / metabolism* Cytoplasm / metabolism* Gene Silencing Genes, Helminth Germ Cells / metabolism Inheritance Patterns / genetics* Open Reading Frames / genetics Protein Binding RNA Interference* RNA, Small Interfering / metabolism Uridine / metabolism |
| IF | 7.815 |
| Times Cited | 10 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 11 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1019 tm2401 tm1021 |