RRC ID 56251
著者 Zhu X, Liu Y, Zhang H, Liu P.
タイトル Whole-genome RNAi screen identifies methylation-related genes influencing lipid metabolism in Caenorhabditis elegans.
ジャーナル J Genet Genomics
Abstract Lipid droplets (LDs) are highly conserved multifunctional cellular organelles and aberrant lipid storage in LDs can lead to many metabolic diseases. However, the molecular mechanisms governing lipid dynamic changes remain elusive, and the high-throughput screen of genes influencing LD morphology was limited by lacking specific LD marker proteins in the powerful genetic tool Caenorhabditis elegans. In this study, we established a new method to conduct whole-genome RNAi screen using LD resident protein DHS-3 as a LD marker, and identified 78 genes involved in significant LD morphologic changes. Among them, mthf-1, as well as a series of methylation-related genes, was found dramatically influencing lipid metabolism. SREBP-1 and SCD1 homologs in C. elegans were involved in the lipid metabolic change of mthf-1(RNAi) worms, and the regulation of ATGL-1 also contributed to it by decreasing triacylglycerol (TAG) hydrolysis. Overall, this study not only identified important genes involved in LD dynamics, but also provided a new tool for LD study using C. elegans, with implications for the study of lipid metabolic diseases.
巻・号 45(5)
ページ 259-272
公開日 2018-5-20
DOI 10.1016/j.jgg.2018.03.005
PII S1673-8527(18)30081-X
PMID 29858166
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* DNA Methylation* Genomics* Lipid Droplets / metabolism* Phenotype RNA Interference*
IF 4.65
引用数 1
リソース情報
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