論文 - 詳細
RRC ID | 56344 |
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著者 | Tsutsui M, Tanaka M, Marui T, Yokota K, Yoshida T, Arima A, Tonomura W, Taniguchi M, Washio T, Okochi M, Kawai T. |
タイトル | Identification of Individual Bacterial Cells through the Intermolecular Interactions with Peptide-Functionalized Solid-State Pores. |
ジャーナル | Anal Chem |
Abstract |
Bioinspired pore sensing for selective detection of flagellated bacteria was investigated. The Au micropore wall surface was modified with a synthetic peptide designed from toll-like receptor 5 (TLR5) to mimic the pathogen-recognition capability. We found that intermolecular interactions between the TLR5-derived recognition peptides and flagella induce ligand-specific perturbations in the translocation dynamics of Escherichia coli, which facilitated the discrimination between the wild-type and flagellin-deletion mutant (ΔfliC) by the resistive pulse patterns thereby demonstrating the sensing of bacteria at a single-cell level. These results provide a novel concept of utilizing weak intermolecular interactions as a recognition probes for single-cell microbial identification. |
巻・号 | 90(3) |
ページ | 1511-1515 |
公開日 | 2018-2-6 |
DOI | 10.1021/acs.analchem.7b04950 |
PMID | 29350898 |
MeSH | Escherichia coli / cytology* Flagellin / chemistry Flagellin / genetics Humans Mutation Peptides / chemistry* Toll-Like Receptor 5 / chemistry* |
IF | 6.785 |
引用数 | 13 |
リソース情報 | |
原核生物(大腸菌) | BW25113 Keio JW1908 |