| RRC ID |
56356
|
| 著者 |
Yamamoto N, Isshiki R, Kawai Y, Tanaka D, Sekiguchi T, Matsumoto S, Tsuneda S.
|
| タイトル |
Stochastic expression of lactate dehydrogenase A induces Escherichia coli persister formation.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
Bacterial persisters are phenotypic variants that survive the treatment of lethal doses of growth-targeting antibiotics without mutations. Although the mechanism underlying persister formation has been studied for decades, how the persister phenotype is switched on and protects itself from antibiotics has been elusive. In this study, we focused on the lactate dehydrogenase gene (ldhA) that was upregulated in an Escherichia coli persister-enriched population. A survival rate assay using an ldhA-overexpressing strain showed that ldhA expression induced persister formation. To identify ldhA-mediated persister formation at the single-cell level, time-lapse microscopy with a microfluidic device was used. Stochastic ldhA expression was found to induce dormancy and tolerance against high-dose ampicillin treatment (500 μg/ml). To better understand the underlying mechanism, we investigated the relationship between ldhA-mediated persister formation and previously reported persister formation through aerobic metabolism repression. As a result, ldhA expression enhanced the proton motive force (PMF) and ATP synthesis. These findings suggest that ldhA-mediated persister formation pathway is different from previously reported persister formation via repression of aerobic metabolism.
|
| 巻・号 |
126(1)
|
| ページ |
30-37
|
| 公開日 |
2018-7-1
|
| DOI |
10.1016/j.jbiosc.2018.01.016
|
| PII |
S1389-1723(17)31004-6
|
| PMID |
29449156
|
| MeSH |
Anti-Bacterial Agents / pharmacology
Drug Resistance, Bacterial / genetics*
Energy Metabolism / drug effects
Energy Metabolism / genetics
Escherichia coli / drug effects
Escherichia coli / genetics*
Escherichia coli / physiology
Escherichia coli Proteins / genetics
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic / physiology
Isoenzymes / genetics
L-Lactate Dehydrogenase / genetics*
Lactate Dehydrogenase 5
Phenotype
Transcriptional Activation / drug effects
|
| IF |
2.032
|
| 引用数 |
3
|
| リソース情報 |
| 原核生物(大腸菌) |
Keio JW1375
Keio JW1908 |
