Reference - Detail
| RRC ID | 56366 |
|---|---|
| Author | Jiang N, Tang L, Xie R, Li Z, Burkinshaw B, Liang X, Sosa D, Aravind L, Dong T, Zhang D, Zheng J. |
| Title | Vibrio parahaemolyticus RhsP represents a widespread group of pro-effectors for type VI secretion systems. |
| Journal | Nat Commun |
| Abstract |
Type VI secretion systems (T6SSs) translocate effector proteins, such as Rhs toxins, to eukaryotic cells or prokaryotic competitors. All T6SS Rhs-type effectors characterized thus far contain a PAAR motif or a similar structure. Here, we describe a T6SS-dependent delivery mechanism for a subset of Rhs proteins that lack a PAAR motif. We show that the N-terminal Rhs domain of protein RhsP (or VP1517) from Vibrio parahaemolyticus inhibits the activity of the C-terminal DNase domain. Upon auto-proteolysis, the Rhs fragment remains inside the cells, and the C-terminal region interacts with PAAR2 and is secreted by T6SS2; therefore, RhsP acts as a pro-effector. Furthermore, we show that RhsP contributes to the control of certain "social cheaters" (opaR mutants). Genes encoding proteins with similar Rhs and PAAR-interacting domains, but diverse C-terminal regions, are widely distributed among Vibrio species. |
| Volume | 9(1) |
| Pages | 3899 |
| Published | 2018-9-25 |
| DOI | 10.1038/s41467-018-06201-5 |
| PII | 10.1038/s41467-018-06201-5 |
| PMID | 30254227 |
| PMC | PMC6156420 |
| MeSH | Amino Acid Motifs / genetics Amino Acid Sequence Bacterial Proteins / chemistry Bacterial Proteins / genetics* Bacterial Proteins / metabolism Bacterial Toxins / chemistry Bacterial Toxins / genetics* Bacterial Toxins / metabolism Binding Sites / genetics Mutagenesis, Site-Directed Protein Binding Protein Domains Sequence Homology, Amino Acid Type VI Secretion Systems / genetics* Type VI Secretion Systems / metabolism Vibrio parahaemolyticus / genetics* Vibrio parahaemolyticus / metabolism |
| IF | 11.878 |
| Times Cited | 7 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 24 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Pathogenic bacteria | JNBP_30766(RIMD 2210633) |