RRC ID 56366
著者 Jiang N, Tang L, Xie R, Li Z, Burkinshaw B, Liang X, Sosa D, Aravind L, Dong T, Zhang D, Zheng J.
タイトル Vibrio parahaemolyticus RhsP represents a widespread group of pro-effectors for type VI secretion systems.
ジャーナル Nat Commun
Abstract Type VI secretion systems (T6SSs) translocate effector proteins, such as Rhs toxins, to eukaryotic cells or prokaryotic competitors. All T6SS Rhs-type effectors characterized thus far contain a PAAR motif or a similar structure. Here, we describe a T6SS-dependent delivery mechanism for a subset of Rhs proteins that lack a PAAR motif. We show that the N-terminal Rhs domain of protein RhsP (or VP1517) from Vibrio parahaemolyticus inhibits the activity of the C-terminal DNase domain. Upon auto-proteolysis, the Rhs fragment remains inside the cells, and the C-terminal region interacts with PAAR2 and is secreted by T6SS2; therefore, RhsP acts as a pro-effector. Furthermore, we show that RhsP contributes to the control of certain "social cheaters" (opaR mutants). Genes encoding proteins with similar Rhs and PAAR-interacting domains, but diverse C-terminal regions, are widely distributed among Vibrio species.
巻・号 9(1)
ページ 3899
公開日 2018-9-25
DOI 10.1038/s41467-018-06201-5
PII 10.1038/s41467-018-06201-5
PMID 30254227
PMC PMC6156420
MeSH Amino Acid Motifs / genetics Amino Acid Sequence Bacterial Proteins / chemistry Bacterial Proteins / genetics* Bacterial Proteins / metabolism Bacterial Toxins / chemistry Bacterial Toxins / genetics* Bacterial Toxins / metabolism Binding Sites / genetics Mutagenesis, Site-Directed Protein Binding Protein Domains Sequence Homology, Amino Acid Type VI Secretion Systems / genetics* Type VI Secretion Systems / metabolism Vibrio parahaemolyticus / genetics* Vibrio parahaemolyticus / metabolism
IF 11.878
引用数 7
リソース情報
病原細菌 JNBP_30766(RIMD 2210633)