論文 - 詳細
| RRC ID | 56368 |
|---|---|
| 著者 | Dar Y, Salomon D, Bosis E. |
| タイトル | The Antibacterial and Anti-Eukaryotic Type VI Secretion System MIX-Effector Repertoire in Vibrionaceae. |
| ジャーナル | Mar Drugs |
| Abstract |
Vibrionaceae is a widespread family of aquatic bacteria that includes emerging pathogens and symbionts. Many Vibrionaceae harbor a type VI secretion system (T6SS), which is a secretion apparatus used to deliver toxins, termed effectors, into neighboring cells. T6SSs mediate both antibacterial and anti-eukaryotic activities. Notably, antibacterial effectors are encoded together with a gene that encodes a cognate immunity protein so as to antagonize the toxicity of the effector. The MIX (Marker for type sIX effectors) domain has been previously defined as a marker of T6SS effectors carrying polymorphic C-terminal toxins. Here, we set out to identify the Vibrionaceae MIX-effector repertoire and to analyze the various toxin domains they carry. We used a computational approach to search for the MIX-effectors in the Vibrionaceae genomes, and grouped them into clusters based on the C-terminal toxin domains. We classified MIX-effectors as either antibacterial or anti-eukaryotic, based on the presence or absence of adjacent putative immunity genes, respectively. Antibacterial MIX-effectors carrying pore-forming, phospholipase, nuclease, peptidoglycan hydrolase, and protease activities were found. Furthermore, we uncovered novel virulence MIX-effectors. These are encoded by "professional MIXologist" strains that employ a cocktail of antibacterial and anti-eukaryotic MIX-effectors. Our findings suggest that certain Vibrionaceae adapted their antibacterial T6SS to mediate interactions with eukaryotic hosts or predators. |
| 巻・号 | 16(11) |
| 公開日 | 2018-11-4 |
| DOI | 10.3390/md16110433 |
| PII | md16110433 |
| PMID | 30400344 |
| PMC | PMC6267618 |
| MeSH | Anti-Bacterial Agents / metabolism Anti-Bacterial Agents / toxicity* Aquatic Organisms / metabolism* Bacterial Proteins / genetics Bacterial Proteins / metabolism Bacterial Proteins / toxicity* Computational Biology Eukaryota / physiology* Host Microbial Interactions / physiology Protein Domains / genetics Type VI Secretion Systems / genetics Type VI Secretion Systems / metabolism Type VI Secretion Systems / toxicity* Vibrionaceae / genetics Vibrionaceae / metabolism* Virulence Factors / genetics Virulence Factors / metabolism Virulence Factors / toxicity |
| IF | 3.772 |
| 引用数 | 4 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 19 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原細菌 | Vibrionaceae. |