RRC ID 5638
Author Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.
Title Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.
Journal Nature
Abstract Genome-wide RNA interference (RNAi) screens have identified near-complete sets of genes involved in cellular processes. However, this methodology has not yet been used to study complex developmental processes in a tissue-specific manner. Here we report the use of a library of Drosophila strains expressing inducible hairpin RNAi constructs to study the Notch signalling pathway during external sensory organ development. We assigned putative loss-of-function phenotypes to 21.2% of the protein-coding Drosophila genes. Using secondary assays, we identified 6 new genes involved in asymmetric cell division and 23 novel genes regulating the Notch signalling pathway. By integrating our phenotypic results with protein interaction data, we constructed a genome-wide, functionally validated interaction network governing Notch signalling and asymmetric cell division. We used clustering algorithms to identify nuclear import pathways and the COP9 signallosome as Notch regulators. Our results show that complex developmental processes can be analysed on a genome-wide level and provide a unique resource for functional annotation of the Drosophila genome.
Volume 458(7241)
Pages 987-92
Published 2009-4-23
DOI 10.1038/nature07936
PII nature07936
PMID 19363474
PMC PMC2988197
MeSH Active Transport, Cell Nucleus Animals Animals, Genetically Modified Cell Division / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism* Genome, Insect / genetics* Phenotype Quality Control RNA Interference* Receptors, Notch / metabolism* Signal Transduction / genetics*
IF 43.07
Times Cited 200
Drosophila 10210R-1 10210R-2 10618R-1 10618R-2 10776R-1 10776R-2 10954R-1 10954R-4 11101R-2 11101R-3 11940R-2 11940R-3 12352R-1 12352R-3 12484R-1 12484R-2 12818R-1 12818R-3 12880R-1 13208R-2 13208R-3 13472R-1 13472R-3 14721R-2 14721R-2 15443R-1 15443R-2 15769R-2 15769R-4 16908R-4 16993R-1 16993R-2 17559R-2 17559R-3 17579R-1 17657R-1 17657R-3 17928R-1 17928R-2 1804R-1 1804R-3 18214R-1 8214R-2 1903R-2 2179R-2 2183R-1 31904R-2 31904R-3 4350R-2 4350R-1 12542R-3 3298R-1 3298R-2 13831R-1 13831R-2 15637R-1 15637R-2 31724R-2 31725R-1 31725R-3 31727R-1 31727R-3 32049R-1 32049R-2 32311R-1 32311R-2 2905R-3 2905R-7 15507R-2 15507R-4 3690R-1 3722R-1 3722R-2 3779R-1 3779R-3 3891R-1 3891R-2 3936R-2 3936R-3 4311R-1 4311R-3 4633R-2 4633R-4 5055R-2 5055R-1 5165R-2 5608R-1 5608R-3 5640R-3 5640R-2 5680R-1 5680R-2 6098R-1 6098R-2 6255R-3 6627R-4 6883R-1 6883R-2 7454R-1 7454R-3 7614R-1 7614R-3 7999R-1 7999R-2 8142R-2 8142R-3 8657R-1 8657R-2 8865R-1 8865R-2 8890R-2 9249R-1 9249R-3 9596R-1 9596R-2 9901R-2 9901R-1