RRC ID 56385
Author Ishida Y, Tsunashima M, Hiei Y, Komari T.
Title Wheat (Triticum aestivum L.) transformation using immature embryos.
Journal Methods Mol. Biol.
Abstract Wheat may now be transformed very efficiently by Agrobacterium tumefaciens. Under the protocol hereby described, immature embryos of healthy plants of wheat cultivar Fielder grown in a well-conditioned greenhouse were pretreated with centrifuging and cocultivated with A. tumefaciens. Transgenic wheat plants were obtained routinely from between 40 and 90 % of the immature embryos, thus infected in our tests. All regenerants were normal in morphology and fully fertile. About half of the transformed plants carried single copy of the transgene, which are inherited by the progeny in a Mendelian fashion.
Volume 1223
Pages 189-98
Published 2015
DOI 10.1007/978-1-4939-1695-5_15
PMID 25300841
MeSH Agriculture / methods Agrobacterium tumefaciens / genetics Cinnamates / pharmacology Coculture Techniques Drug Resistance / genetics Genetic Techniques* Hygromycin B / analogs & derivatives Hygromycin B / pharmacology Plants, Genetically Modified* Seeds / genetics* Seeds / growth & development Selection, Genetic Transformation, Bacterial Triticum / drug effects Triticum / genetics* Triticum / growth & development
Resource
Wheat KT020-061