論文 - 詳細
| RRC ID | 56385 |
|---|---|
| 著者 | Ishida Y, Tsunashima M, Hiei Y, Komari T. |
| タイトル | Wheat (Triticum aestivum L.) transformation using immature embryos. |
| ジャーナル | Methods Mol Biol |
| Abstract |
Wheat may now be transformed very efficiently by Agrobacterium tumefaciens. Under the protocol hereby described, immature embryos of healthy plants of wheat cultivar Fielder grown in a well-conditioned greenhouse were pretreated with centrifuging and cocultivated with A. tumefaciens. Transgenic wheat plants were obtained routinely from between 40 and 90 % of the immature embryos, thus infected in our tests. All regenerants were normal in morphology and fully fertile. About half of the transformed plants carried single copy of the transgene, which are inherited by the progeny in a Mendelian fashion. |
| 巻・号 | 1223 |
| ページ | 189-98 |
| 公開日 | 2015-1-1 |
| DOI | 10.1007/978-1-4939-1695-5_15 |
| PMID | 25300841 |
| MeSH | Agriculture / methods Agrobacterium tumefaciens / genetics Cinnamates / pharmacology Coculture Techniques Drug Resistance / genetics Genetic Techniques* Hygromycin B / analogs & derivatives Hygromycin B / pharmacology Plants, Genetically Modified* Seeds / genetics* Seeds / growth & development Selection, Genetic Transformation, Bacterial Triticum / drug effects Triticum / genetics* Triticum / growth & development |
| 引用数 | 65 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 22 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 6.0 |
| リソース情報 | |
| コムギ | KT020-061 |