RRC ID 56577
Author Danilova TV, Friebe B, Gill BS, Poland J, Jackson E.
Title Chromosome Rearrangements Caused by Double Monosomy in Wheat-Barley Group-7 Substitution Lines.
Journal Cytogenet Genome Res
Abstract Interspecific or introgressive hybridization is one of the driving forces in plant speciation, producing allopolyploids or diploids with rearranged genomes. The process of karyotype reshaping following homoploid interspecific hybridization has not been studied experimentally. Interspecific hybridization is widely used in plant breeding to increase genetic diversity and introgress new traits. Numerous introgression stocks were developed for hexaploid wheat Triticum aestivum L. (2n = 6x = 42, genome AABBDD). Double monosomic lines, containing one alien chromosome from the tertiary gene pool of wheat and one homoeologous wheat chromosome, represent a simplified model for studying chromosome rearrangements caused by interspecific hybridization. The pairing of a chromosome from the tertiary gene pool with a wheat homoeologue is restricted by the activity of the wheat Ph1 gene, thus, rearrangements caused by chromosome breakage followed by the fusion of the broken arms can be expected. We analyzed chromosome aberrations in 4 sets of lines that originated from double monosomics of barley (Hordeum vulgare L.) chromosome 7H and wheat group-7 chromosomes with dicentric or ring chromosomes. The dynamics of wheat-barley dicentric chromosomes during plant development was followed and an increased diversity of rearrangements was observed. Besides the targeted group-7 chromosomes, other wheat chromosomes were involved in rearrangements, as chromosomes broken in the centromeric region fused with other broken chromosomes. In some cells, multi-centric chromosomes were observed. The structure and dosage of the introgressed barley chromatin was changed. The transmission of the rearrangements to the progenies was analyzed. The observed aberrations emphasize the importance of cytogenetic screening in gene introgression projects.
Volume 154(1)
Pages 45-55
Published 2018-2-28
DOI 10.1159/000487183
PII 000487183
PMID 29486464
MeSH Chromosome Aberrations Chromosomes, Plant / genetics* Genetic Speciation Hordeum / genetics* Hybridization, Genetic Karyotyping Monosomy Triticum / genetics*
IF 1.423
Times Cited 4
Wheat tplb0013b07 tplb0007o14 tplb0015e09