Reference - Detail
| RRC ID | 565 |
|---|---|
| Author | Ohsaki A, Iiyama K, Miyagawa Y, Kawaguchi Y, Koga K, Kusakabe T. |
| Title | Nonhomologous end-joining in a cell-free extract from the cultured silkworm cell line BmN4. |
| Journal | Mol Biol Rep |
| Abstract |
Nonhomologous end-joining (NHEJ) is one of the repair pathways for double-strand breaks (DSBs) in eukaryotic cells. By using linearized plasmid substrates, we have detected intramolecular NHEJ activity in a cell-free extract from the cultured silkworm cell line BmN4. The efficiency of NHEJ differed according to the structure of DNA ends; approximately 1% of input DNA was repaired when the substrate had cohesive ends. The reaction required the hydrolysis of nucleotide triphosphate; interestingly, all of four rNTPs or four dNTPs could support the reaction. A substrate with non-complementary DNA ends was mainly repaired by the DNA polymerase-mediated pathway. These results indicate that the present cell-free system will be useful to analyze the molecular mechanisms of DSB repair and NHEJ in insect cells. |
| Volume | 32(1) |
| Pages | 25-34 |
| Published | 2005-3-1 |
| DOI | 10.1007/s11033-004-2474-y |
| PMID | 15865207 |
| MeSH | Animals Bombyx / chemistry Bombyx / cytology Bombyx / genetics* Cations, Divalent / chemistry Cell Line Cell-Free System DNA Damage* DNA Repair* Metals / chemistry Plasmids / chemistry Plasmids / genetics Recombination, Genetic |
| IF | 1.402 |
| Times Cited | 2 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Silkworms | |