RRC ID 56630
Author Seike T, Maekawa H, Nakamura T, Shimoda C.
Title The asymmetric chemical structures of two mating pheromones reflect their differential roles in mating of fission yeast.
Journal J Cell Sci
Abstract In the fission yeast Schizosaccharomyces pombe, the mating reaction is controlled by two mating pheromones, M-factor and P-factor, secreted by M- and P-type cells, respectively. M-factor is a C-terminally farnesylated lipid peptide, whereas P-factor is a simple peptide. To examine whether this chemical asymmetry in the two pheromones is essential for conjugation, we constructed a mating system in which either pheromone can stimulate both M- and P-cells, and examined whether the resulting autocrine strains can mate. Autocrine M-cells responding to M-factor successfully mated with P-factor-lacking P-cells, indicating that P-factor is not essential for conjugation; by contrast, autocrine P-cells responding to P-factor were unable to mate with M-factor-lacking M-cells. The sterility of the autocrine P-cells was completely restored by expressing the M-factor receptor. These observations indicate that the different chemical characteristics of the two types of pheromone, a lipid and a simple peptide, are not essential; however, a lipid peptide might be required for successful mating. Our findings allow us to propose a model of the differential roles of M-factor and P-factor in conjugation of S. pombeThis article has an associated First Person interview with the first author of the paper.
Volume 132(12)
Published 2019-6-25
DOI 10.1242/jcs.230722
PII jcs.230722
PMID 31186279
IF 4.517
Times Cited 1
Resource
Yeast FY25441(FS65) FY25442(FS66) FY7507(L972) FY7519(L975)