RRC ID 56692
Author Tsutsui H, Sakatsume O, Itakura K, Yokoyama KK.
Title Members of the MAZ family: a novel cDNA clone for MAZ from human pancreatic islet cells.
Journal Biochem Biophys Res Commun
Abstract A human recombinant cDNA clone that encoded a zinc-finger protein (Myc-associated zinc-finger protein of human islet; MAZi) was cloned by screening a cDNA library prepared from human pancreatic islet cells. The encoded protein showed a high degree of homology to the Myc-associated zinc-finger protein MAZ (ZF87 or Pur-1). However, differences between the cDNAs for MAZi and MAZ were found in the length of the encoded polyalanine stretch and in the sequence of the 5'-end leader. MAZi transcripts were significantly more abundant in rat pancreatic islet carcinoma tissue than in normal rat islet cells. Moreover, MAZi protein bound specifically to the pyrimidine-rich strand of the CT-element of the c-myc gene in vitro and strongly induced the expression of chloramphenicol acetyltransferase (CAT) from a c-myc promoter/ CAT reporter construct in human pancreatic cells. Our results suggest that a distinct member of the MAZ family is expressed in human islet cells and enhances the transcriptional activity of the c-myc gene.
Volume 226(3)
Pages 801-9
Published 1996-9-24
DOI 10.1006/bbrc.1996.1432
PII S0006-291X(96)91432-1
PMID 8831693
MeSH Amino Acid Sequence Animals Base Sequence Cell Line Cells, Cultured Chloramphenicol O-Acetyltransferase / biosynthesis Cloning, Molecular DNA Probes DNA, Complementary DNA-Binding Proteins / isolation & purification DNA-Binding Proteins / metabolism Genes, Reporter Humans Insulinoma Islets of Langerhans / metabolism* Molecular Sequence Data Pancreatic Neoplasms Protein Structure, Secondary Rats Recombinant Proteins / biosynthesis Recombinant Proteins / chemistry Recombinant Proteins / isolation & purification Transcription Factors / biosynthesis* Transcription Factors / chemistry Transcription Factors / isolation & purification Zinc Fingers
IF 2.705
Times Cited 24
DNA material pCMV-MAZ (RDB03219) pCMV-MAZ (RDB04788)