論文 - 詳細
| RRC ID | 56692 |
|---|---|
| 著者 | Tsutsui H, Sakatsume O, Itakura K, Yokoyama KK. |
| タイトル | Members of the MAZ family: a novel cDNA clone for MAZ from human pancreatic islet cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
A human recombinant cDNA clone that encoded a zinc-finger protein (Myc-associated zinc-finger protein of human islet; MAZi) was cloned by screening a cDNA library prepared from human pancreatic islet cells. The encoded protein showed a high degree of homology to the Myc-associated zinc-finger protein MAZ (ZF87 or Pur-1). However, differences between the cDNAs for MAZi and MAZ were found in the length of the encoded polyalanine stretch and in the sequence of the 5'-end leader. MAZi transcripts were significantly more abundant in rat pancreatic islet carcinoma tissue than in normal rat islet cells. Moreover, MAZi protein bound specifically to the pyrimidine-rich strand of the CT-element of the c-myc gene in vitro and strongly induced the expression of chloramphenicol acetyltransferase (CAT) from a c-myc promoter/ CAT reporter construct in human pancreatic cells. Our results suggest that a distinct member of the MAZ family is expressed in human islet cells and enhances the transcriptional activity of the c-myc gene. |
| 巻・号 | 226(3) |
| ページ | 801-9 |
| 公開日 | 1996-9-24 |
| DOI | 10.1006/bbrc.1996.1432 |
| PII | S0006-291X(96)91432-1 |
| PMID | 8831693 |
| MeSH | Amino Acid Sequence Animals Base Sequence Cell Line Cells, Cultured Chloramphenicol O-Acetyltransferase / biosynthesis Cloning, Molecular DNA Probes DNA, Complementary DNA-Binding Proteins / isolation & purification DNA-Binding Proteins / metabolism Genes, Reporter Humans Insulinoma Islets of Langerhans / metabolism* Molecular Sequence Data Pancreatic Neoplasms Protein Structure, Secondary Rats Recombinant Proteins / biosynthesis Recombinant Proteins / chemistry Recombinant Proteins / isolation & purification Transcription Factors / biosynthesis* Transcription Factors / chemistry Transcription Factors / isolation & purification Zinc Fingers |
| IF | 2.705 |
| 引用数 | 24 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCMV-MAZ (RDB03219) pCMV-MAZ (RDB04788) |