| RRC ID |
56692
|
| 著者 |
Tsutsui H, Sakatsume O, Itakura K, Yokoyama KK.
|
| タイトル |
Members of the MAZ family: a novel cDNA clone for MAZ from human pancreatic islet cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
A human recombinant cDNA clone that encoded a zinc-finger protein (Myc-associated zinc-finger protein of human islet; MAZi) was cloned by screening a cDNA library prepared from human pancreatic islet cells. The encoded protein showed a high degree of homology to the Myc-associated zinc-finger protein MAZ (ZF87 or Pur-1). However, differences between the cDNAs for MAZi and MAZ were found in the length of the encoded polyalanine stretch and in the sequence of the 5'-end leader. MAZi transcripts were significantly more abundant in rat pancreatic islet carcinoma tissue than in normal rat islet cells. Moreover, MAZi protein bound specifically to the pyrimidine-rich strand of the CT-element of the c-myc gene in vitro and strongly induced the expression of chloramphenicol acetyltransferase (CAT) from a c-myc promoter/ CAT reporter construct in human pancreatic cells. Our results suggest that a distinct member of the MAZ family is expressed in human islet cells and enhances the transcriptional activity of the c-myc gene.
|
| 巻・号 |
226(3)
|
| ページ |
801-9
|
| 公開日 |
1996-9-24
|
| DOI |
10.1006/bbrc.1996.1432
|
| PII |
S0006-291X(96)91432-1
|
| PMID |
8831693
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Cell Line
Cells, Cultured
Chloramphenicol O-Acetyltransferase / biosynthesis
Cloning, Molecular
DNA Probes
DNA, Complementary
DNA-Binding Proteins / isolation & purification
DNA-Binding Proteins / metabolism
Genes, Reporter
Humans
Insulinoma
Islets of Langerhans / metabolism*
Molecular Sequence Data
Pancreatic Neoplasms
Protein Structure, Secondary
Rats
Recombinant Proteins / biosynthesis
Recombinant Proteins / chemistry
Recombinant Proteins / isolation & purification
Transcription Factors / biosynthesis*
Transcription Factors / chemistry
Transcription Factors / isolation & purification
Zinc Fingers
|
| IF |
2.705
|
| 引用数 |
24
|
| リソース情報 |
| 遺伝子材料 |
pCMV-MAZ (RDB03219)
pCMV-MAZ (RDB04788) |
