| RRC ID |
56727
|
| Author |
Terakawa M, Sato S, Ashida H, Aizawa K, Uenoyama M, Masaki Y, Obara M.
|
| Title |
In vitro gene transfer to mammalian cells by the use of laser-induced stress waves: effects of stress wave parameters, ambient temperature, and cell type.
|
| Journal |
J Biomed Opt
|
| Abstract |
Laser-mediated gene transfection has received much attention as a new method for targeted gene therapy because of the high spatial controllability of laser energy. We previously demonstrated both in vivo and in vitro that plasmid DNA can be transfected by applying nanosecond pulsed laser-induced stress waves (LISWs). In the present study, we investigated the dependence of transfection efficiency on the laser irradiation conditions and hence stress wave conditions in vitro. We measured characteristics of LISWs used for gene transfection. For NIH 3T3 cells, transfection efficiency was evaluated as functions of laser fluence and number of pulses. The effect of ambient temperature was also investigated, and it was found that change in ambient temperature in a specific range resulted in drastic change in transfection efficiency for NIH 3T3 cells. Gene transfection of different types of cell lines were also demonstrated, where cellular heating increased transfection efficiency for nonmalignant cells, while heating decreased transfection efficiency for malignant cells.
|
| Volume |
11(1)
|
| Pages |
014026
|
| Published |
2006-1-1
|
| DOI |
10.1117/1.2160407
|
| PMID |
16526903
|
| MeSH |
Animals
CHO Cells
Cell Membrane / metabolism*
Cell Membrane / radiation effects*
Cell Membrane Permeability / radiation effects
Cells, Cultured
Cricetinae
Cricetulus
Dose-Response Relationship, Radiation
Fibroblasts / metabolism*
Fibroblasts / radiation effects
HeLa Cells
Humans
Lasers*
Mice
NIH 3T3 Cells
Plasmids / genetics*
Plasmids / pharmacokinetics*
Radiation Dosage
Species Specificity
Stress, Mechanical
Temperature
Transfection / methods*
|
| IF |
2.785
|
| Times Cited |
13
|
| Resource |
| Human and Animal Cells |
NIH3T3-3-4(RCB1862)
HeLa(RCB0007)
CHO-K1(RCB0285) |
