Reference - Detail
|Author||Okino Y, Inayoshi Y, Kojima Y, Kidani S, Kaneoka H, Honkawa A, Higuchi H, Nishijima K, Miyake K, Iijima S.|
|Title||Moloney murine leukemia virus integrase and reverse transcriptase interact with PML proteins.|
Pull-down assay and co-immunoprecipitation of cell extracts in which the integrase or reverse transcriptase of Moloney murine leukemia virus was transiently expressed showed that both enzymes interacted with PML proteins. In infected cells, interaction between the integrase and PML was also observed. Transient expression of PIASy and SUMO proteins facilitated SUMOylation of the integrase but had no apparent effects on the interaction with PML. A FLAG-tagged integrase co-localized with PML protein possibly in the PML body. Knockdown of PML by small interfering RNA resulted in reduced viral cDNA levels and integration efficiency. This suggested that PML proteins activated reverse transcription.
|MeSH||Animals Cell Nucleus Structures / metabolism Gene Knockdown Techniques HeLa Cells Host-Pathogen Interactions Humans Immunoprecipitation Integrases / genetics Integrases / metabolism* Mice Moloney murine leukemia virus / enzymology* NIH 3T3 Cells / virology Nuclear Proteins / genetics Nuclear Proteins / metabolism* Promyelocytic Leukemia Protein RNA-Directed DNA Polymerase / genetics RNA-Directed DNA Polymerase / metabolism* SUMO-1 Protein / genetics SUMO-1 Protein / metabolism Small Ubiquitin-Related Modifier Proteins / genetics Small Ubiquitin-Related Modifier Proteins / metabolism Transcription Factors / genetics Transcription Factors / metabolism* Tumor Suppressor Proteins / genetics Tumor Suppressor Proteins / metabolism*|
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