Reference - Detail
| RRC ID | 56901 |
|---|---|
| Author | Yamamoto K, Yamada N. |
| Title | Identification of a diazinon-metabolizing glutathione S-transferase in the silkworm, Bombyx mori. |
| Journal | Sci Rep |
| Abstract |
The glutathione S-transferase superfamily play key roles in the metabolism of numerous xenobiotics. We report herein the identification and characterization of a novel glutathione S-transferase in the silkworm, Bombyx mori. The enzyme (bmGSTu2) conjugates glutathione to 1-chloro-2,4-dinitrobenzene, as well as metabolizing diazinon, one of the organophosphate insecticides. Quantitative reverse transcription-polymerase chain reaction analysis of transcripts demonstrated that bmGSTu2 expression was induced 1.7-fold in a resistant strain of B. mori. Mutagenesis of putative amino acid residues in the glutathione-binding site revealed that Ile54, Glu66, Ser67, and Asn68 are crucial for enzymatic function. These results provide insights into the catalysis of glutathione conjugation in silkworm by bmGSTu2 and into the detoxification of organophosphate insecticides. |
| Volume | 6 |
| Pages | 30073 |
| Published | 2016-7-21 |
| DOI | 10.1038/srep30073 |
| PII | srep30073 |
| PMID | 27440377 |
| PMC | PMC4954967 |
| MeSH | Animals Binding Sites Biotransformation Bombyx / enzymology* DNA Mutational Analysis Diazinon / metabolism* Dinitrochlorobenzene / metabolism Gene Expression Profiling Glutathione / metabolism Glutathione Transferase / genetics Glutathione Transferase / metabolism* Insecticides / metabolism* |
| IF | 4.011 |
| Times Cited | 15 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Silkworms | |