RRC ID 56921
Author Sumitani M, Kondo M, Kasashima K, Endo H, Nakamura K, Misawa T, Tanaka H, Sezutsu H.
Title Characterization of Bombyx mori mitochondrial transcription factor A, a conserved regulator of mitochondrial DNA.
Journal Gene
Abstract In the present study, we initially cloned and characterized a mitochondrial transcription factor A (Tfam) homologue in the silkworm, Bombyx mori. Bombyx mori TFAM (BmTFAM) localized to mitochondria in cultured silkworm and human cells, and co-localized with mtDNA nucleoids in human HeLa cells. In an immunoprecipitation analysis, BmTFAM was found to associate with human mtDNA in mitochondria, indicating its feature as a non-specific DNA-binding protein. In spite of the low identity between BmTFAM and human TFAM (26.5%), the expression of BmTFAM rescued mtDNA copy number reductions and enlarged mtDNA nucleoids in HeLa cells, which were induced by human Tfam knockdown. Thus, BmTFAM compensates for the function of human TFAM in HeLa cells, demonstrating that the mitochondrial function of TFAM is highly conserved between silkworms and humans. BmTfam mRNA was strongly expressed in early embryos. Through double-stranded RNA (dsRNA)-based RNA interference (RNAi) in silkworm embryos, we found that the knockdown of BmTFAM reduced the amount of mtDNA and induced growth retardation at the larval stage. Collectively, these results demonstrate that BmTFAM is a highly conserved mtDNA regulator and may be a good candidate for investigating and modulating mtDNA metabolism in this model organism.
Volume 608
Pages 103-113
Published 2017-4-15
DOI 10.1016/j.gene.2016.12.021
PII S0378-1119(16)31021-6
PMID 28027964
MeSH Amino Acid Sequence Animals Bombyx / embryology Bombyx / genetics* Cells, Cultured Cloning, Molecular DNA, Mitochondrial / genetics* DNA-Binding Proteins / genetics* DNA-Binding Proteins / physiology* Gene Expression Regulation, Developmental HeLa Cells Humans Mitochondrial Proteins / genetics* Mitochondrial Proteins / physiology* Sequence Analysis, DNA Transcription Factors / genetics* Transcription Factors / physiology*
IF 2.638
Times Cited 2
Silkworms w1-pnd