RRC ID 56925
Author Moriyama T, Suzuki T, Chang MO, Kitajima M, Takaku H.
Title Baculovirus directly activates murine NK cells via TLR9.
Journal Cancer Gene Ther
Abstract The importance of natural killer (NK) cells in innate immune responses against tumors or viral infections enhances the appeal of NK cell-based immunotherapeutic approaches. We have recently reported that baculovirus (BV)-infected dendritic cells (DCs; BV-DCs) induce antitumor immunity against established tumors in mice. These antitumor effects were CD8+ T-cell and NK cell dependent; however, they were found to be CD4+ T-cell independent. In this study, we investigated the involvement of Toll-like receptor 9 (TLR9) in the process of BV recognition by NK cells. We found that BV directly stimulated NK cells, induced the expression of the activation marker CD69 and promoted interferon-gamma (IFN-γ) production and cytotoxicity. Moreover, TLR9 knockout in mice (tlr9-/- NK cells) inhibited NK cell responses to BV, indicating that TLR9 may have a relevant role in the BV-induced upregulation of NK cell functions. Our data demonstrated for the first time that NK cells directly recognize BV via TLR9, which provides opportunities for the use of this technique as an effective tool for BV-based immunotherapies against malignancies.
Volume 24(4)
Pages 175-179
Published 2017-4
DOI 10.1038/cgt.2017.2
PII cgt20172
PMID 28186087
MeSH Animals Antigens, CD / genetics Antigens, CD / immunology Antigens, Differentiation, T-Lymphocyte / genetics Antigens, Differentiation, T-Lymphocyte / immunology Baculoviridae / immunology* Female Immunotherapy Interferon-gamma / genetics Interferon-gamma / immunology Killer Cells, Natural / immunology* Lectins, C-Type / genetics Lectins, C-Type / immunology Lymphocyte Activation / genetics Lymphocyte Activation / immunology* Mice Mice, Knockout Neoplasms / genetics Neoplasms / immunology Neoplasms / therapy Toll-Like Receptor 9 / genetics Toll-Like Receptor 9 / immunology*
IF 4.681
Times Cited 6
Human and Animal Cells YAC-1