| RRC ID |
56925
|
| 著者 |
Moriyama T, Suzuki T, Chang MO, Kitajima M, Takaku H.
|
| タイトル |
Baculovirus directly activates murine NK cells via TLR9.
|
| ジャーナル |
Cancer Gene Ther
|
| Abstract |
The importance of natural killer (NK) cells in innate immune responses against tumors or viral infections enhances the appeal of NK cell-based immunotherapeutic approaches. We have recently reported that baculovirus (BV)-infected dendritic cells (DCs; BV-DCs) induce antitumor immunity against established tumors in mice. These antitumor effects were CD8+ T-cell and NK cell dependent; however, they were found to be CD4+ T-cell independent. In this study, we investigated the involvement of Toll-like receptor 9 (TLR9) in the process of BV recognition by NK cells. We found that BV directly stimulated NK cells, induced the expression of the activation marker CD69 and promoted interferon-gamma (IFN-γ) production and cytotoxicity. Moreover, TLR9 knockout in mice (tlr9-/- NK cells) inhibited NK cell responses to BV, indicating that TLR9 may have a relevant role in the BV-induced upregulation of NK cell functions. Our data demonstrated for the first time that NK cells directly recognize BV via TLR9, which provides opportunities for the use of this technique as an effective tool for BV-based immunotherapies against malignancies.
|
| 巻・号 |
24(4)
|
| ページ |
175-179
|
| 公開日 |
2017-4-1
|
| DOI |
10.1038/cgt.2017.2
|
| PII |
cgt20172
|
| PMID |
28186087
|
| MeSH |
Animals
Antigens, CD / genetics
Antigens, CD / immunology
Antigens, Differentiation, T-Lymphocyte / genetics
Antigens, Differentiation, T-Lymphocyte / immunology
Baculoviridae / immunology*
Female
Immunotherapy
Interferon-gamma / genetics
Interferon-gamma / immunology
Killer Cells, Natural / immunology*
Lectins, C-Type / genetics
Lectins, C-Type / immunology
Lymphocyte Activation / genetics
Lymphocyte Activation / immunology*
Mice
Mice, Knockout
Neoplasms / genetics
Neoplasms / immunology
Neoplasms / therapy
Toll-Like Receptor 9 / genetics
Toll-Like Receptor 9 / immunology*
|
| IF |
4.681
|
| 引用数 |
6
|
| リソース情報 |
| ヒト・動物細胞 |
YAC-1 |
