RRC ID 5698
Author Niwa R, Nagata-Ohashi K, Takeichi M, Mizuno K, Uemura T.
Title Control of actin reorganization by Slingshot, a family of phosphatases that dephosphorylate ADF/cofilin.
Journal Cell
Abstract The ADF (actin-depolymerizing factor)/cofilin family is a stimulus-responsive mediator of actin dynamics. In contrast to the mechanisms of inactivation of ADF/cofilin by kinases such as LIM-kinase 1 (LIMK1), much less is known about its reactivation through dephosphorylation. Here we report Slingshot (SSH), a family of phosphatases that have the property of F actin binding. In Drosophila, loss of ssh function dramatically increased levels of both F actin and phospho-cofilin (P cofilin) and disorganized epidermal cell morphogenesis. In mammalian cells, human SSH homologs (hSSHs) suppressed LIMK1-induced actin reorganization. Furthermore, SSH and the hSSHs dephosphorylated P cofilin in cultured cells and in cell-free assays. Our results strongly suggest that the SSH family plays a pivotal role in actin dynamics by reactivating ADF/cofilin in vivo.
Volume 108(2)
Pages 233-46
Published 2002-1-25
DOI 10.1016/s0092-8674(01)00638-9
PII S0092867401006389
PMID 11832213
MeSH Actin Depolymerizing Factors Actins / metabolism* Amino Acid Sequence Animals Cell Line Cell-Free System Destrin Drosophila / anatomy & histology Drosophila / genetics Drosophila / metabolism* Drosophila Proteins* Embryonic Structures / anatomy & histology Embryonic Structures / metabolism Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Humans Immunohistochemistry LIM-Homeodomain Proteins Microfilament Proteins / metabolism* Molecular Sequence Data Phosphoprotein Phosphatases Phosphoric Monoester Hydrolases / genetics Phosphoric Monoester Hydrolases / metabolism* Phosphorylation Protein Binding Protein Serine-Threonine Kinases / metabolism Sequence Alignment Transcription Factors Zyxin
IF 38.637
Times Cited 475