RRC ID 56991
Author Hikida H, Kokusho R, Kobayashi J, Shimada T, Katsuma S.
Title Inhibitory role of the Bm8 protein in the propagation of Bombyx mori nucleopolyhedrovirus.
Journal Virus Res.
Abstract Lepidopteran nucleopolyhedroviruses have distinct viral tissue tropisms in host larvae. We previously identified the Bm8 gene of Bombyx mori nucleopolyhedrovirus (BmNPV), the product of which inhibits viral propagation in the middle silk gland (MSG). However, it is unknown whether this inhibitory function of the Bm8 protein is specific to MSGs. Here we generated a Bm8-disrupted recombinant BmNPV expressing green fluorescent protein (GFP) and examined viral propagation in B. mori cultured cells and larvae. We found that Bm8-disrupted BmNPV produced fewer budded viruses and more occlusion bodies (OBs) than the wild-type virus in both cultured cells and larvae. Microscopic observation of OB production and GFP expression revealed that Bm8 disruption accelerated the progression of viral infection in various larval tissues. Furthermore, quantitative reverse transcription-polymerase chain reaction experiments showed that the loss of Bm8 enhanced viral gene expression in BmNPV-infected larval tissues. These results indicate that the Bm8 protein suppresses viral propagation to varying degrees in each larval tissue, which may establish BmNPV tissue tropisms in B. mori larvae.
Volume 249
Pages 124-131
Published 2018-4-2
DOI 10.1016/j.virusres.2018.03.011
PII S0168-1702(17)30774-8
PMID 29574100
MeSH Animals Bombyx / growth & development Bombyx / virology* Cell Line Gene Knockout Techniques Genes, Reporter Green Fluorescent Proteins / analysis Green Fluorescent Proteins / genetics Inclusion Bodies Larva / virology Mutagenesis, Insertional Nucleopolyhedrovirus / genetics Nucleopolyhedrovirus / physiology* Staining and Labeling Viral Proteins / genetics Viral Proteins / metabolism* Virus Replication*
IF 2.484
Resource
Silkworms BmN4 cell