RRC ID 57047
Author Kidani S, Kaneoka H, Okuzaki Y, Asai S, Kojima Y, Nishijima K, Iijima S.
Title Analyses of chicken sialyltransferases related to O-glycosylation.
Journal J Biosci Bioeng
Abstract The chicken β-galactoside α2,3-sialyltransferase 1, 2, and 5 (ST3Gal1, 2, and 5) genes were cloned, and their enzymes were expressed in 293FT cells. ST3Gal1 and 2 exhibited enzymatic activities toward galactose-β1,3-N-acetylgalactosamine and galactose-β1,3-N-acetylglucosamine. ST3Gal5 only exhibited activity toward lactosylceramide. ST3Gal1 and 2 and previously cloned ST3Gal3 and 6 transferred CMP-sialic acid to asialofetuin. Reverse-transcription-quantitative PCR indicated that ST3Gal1 was expressed at higher levels in the trachea, lung, spleen, and magnum, and the strong expression of ST3Gal5 was observed in the spleen, magnum, and small and large intestines. ST3Gal1, 5, and 6 were also expressed in the tubular gland cells of the magnum, which secretes egg-white proteins. ST3Gal1, 5, and 6 were expressed in the egg chorioallantoic membrane, in which influenza viruses are propagated for the production of vaccines.
Volume 122(4)
Pages 379-84
Published 2016-10-1
DOI 10.1016/j.jbiosc.2016.03.017
PII S1389-1723(16)30039-1
PMID 27150510
MeSH Acetylgalactosamine / metabolism Acetylglucosamine / metabolism Animals Antigens, CD / metabolism Asialoglycoproteins / metabolism Cell Line Chickens / genetics* Chorioallantoic Membrane / metabolism Egg Proteins / metabolism Fetuins / metabolism Galactose / metabolism Glycosylation Lactosylceramides / metabolism Organ Specificity Recombinant Proteins / analysis Recombinant Proteins / genetics Recombinant Proteins / metabolism Sialyltransferases / analysis Sialyltransferases / genetics* Sialyltransferases / metabolism* Substrate Specificity
IF 2.366
Times Cited 0
Chicken / Quail