RRC ID 57067
Author Yang SJ, Choi SJ, Park BR, Kim YM.
Title Thermostable CITase from Thermoanaerobacter thermocopriae shows negative cooperativity.
Journal Biotechnol Lett
Abstract OBJECTIVE:The biochemical properties of a putative thermostable cycloisomaltooligosaccharide (CI) glucanotransferase gene from Thermoanaerobacter thermocopriae were determined using a recombinant protein (TtCITase) expressed in Escherichia coli and purified to a single protein.
RESULTS:The 171-kDa protein displayed maximum activity at pH 6.0, and enzyme activity was stable at pH 5.0-11.0. The optimal temperature was 60 °C in 1 h incubation, and thermal stability of the protein was 63% at 60 °C for 24 h. TtCITase produced CI-7 to CI-17, as well as CI-18, CI-19, and CI-20, which are relatively large CIs. Additionally, an unusual kinetic feature of TtCITase was its negative cooperative behavior in the dextran T2000 cleavage reaction.
CONCLUSIONS:Based on our results, TtCITase can be applied to produce relatively large CIs at high temperature.
Volume 41(4-5)
Pages 625-632
Published 2019-5-1
DOI 10.1007/s10529-019-02666-6
PII 10.1007/s10529-019-02666-6
PMID 30927134
MeSH Dextrans / metabolism* Enzyme Stability Escherichia coli / genetics Escherichia coli / metabolism Glucosyltransferases / chemistry Glucosyltransferases / genetics Glucosyltransferases / isolation & purification Glucosyltransferases / metabolism* Hydrogen-Ion Concentration Kinetics Molecular Weight Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Recombinant Proteins / metabolism* Temperature Thermoanaerobacter / enzymology*
IF 2.154
Times Cited 1
DNA material Genomic DNA of Thermoanaerobacter thermocopriaeJCM 7501 T (JGD12533)