Reference - Detail
RRC ID | 57067 |
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Author | Yang SJ, Choi SJ, Park BR, Kim YM. |
Title | Thermostable CITase from Thermoanaerobacter thermocopriae shows negative cooperativity. |
Journal | Biotechnol Lett |
Abstract |
OBJECTIVE:The biochemical properties of a putative thermostable cycloisomaltooligosaccharide (CI) glucanotransferase gene from Thermoanaerobacter thermocopriae were determined using a recombinant protein (TtCITase) expressed in Escherichia coli and purified to a single protein. RESULTS:The 171-kDa protein displayed maximum activity at pH 6.0, and enzyme activity was stable at pH 5.0-11.0. The optimal temperature was 60 °C in 1 h incubation, and thermal stability of the protein was 63% at 60 °C for 24 h. TtCITase produced CI-7 to CI-17, as well as CI-18, CI-19, and CI-20, which are relatively large CIs. Additionally, an unusual kinetic feature of TtCITase was its negative cooperative behavior in the dextran T2000 cleavage reaction. CONCLUSIONS:Based on our results, TtCITase can be applied to produce relatively large CIs at high temperature. |
Volume | 41(4-5) |
Pages | 625-632 |
Published | 2019-5-1 |
DOI | 10.1007/s10529-019-02666-6 |
PII | 10.1007/s10529-019-02666-6 |
PMID | 30927134 |
MeSH | Dextrans / metabolism* Enzyme Stability Escherichia coli / genetics Escherichia coli / metabolism Glucosyltransferases / chemistry Glucosyltransferases / genetics Glucosyltransferases / isolation & purification Glucosyltransferases / metabolism* Hydrogen-Ion Concentration Kinetics Molecular Weight Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Recombinant Proteins / metabolism* Temperature Thermoanaerobacter / enzymology* |
IF | 2.154 |
Times Cited | 1 |
Resource | |
DNA material | Genomic DNA of Thermoanaerobacter thermocopriaeJCM 7501 T (JGD12533) |