論文 - 詳細
| RRC ID | 57107 |
|---|---|
| 著者 | Xu H, Stewart B. |
| タイトル | Imaging SNAP-29 in Drosophila. |
| ジャーナル | Methods Mol Biol |
| Abstract |
SNAP-29 is expressed throughout the life cycle of fruit fly and exhibits wide tissue distribution patterns. Unlike other SNAP-25-like proteins (i.e., SNAP-25, SNAP-23/24, and SNAP-47) which primarily support exocytosis at the plasma membrane, SNAP-29 regulates various intracellular trafficking events, by partnering with proteins active in both exocytosis and endocytosis. Here we describe the protocol to localize SNAP-29 in early embryos, imaginal discs from third instar larva, and immortalized S2 cells via immunofluorescence microscopy. |
| 巻・号 | 1860 |
| ページ | 391-401 |
| 公開日 | 2018-10-14 |
| DOI | 10.1007/978-1-4939-8760-3_26 |
| PMID | 30317520 |
| MeSH | Animals Cell Line Cell Membrane / metabolism* Drosophila Proteins / chemistry Drosophila Proteins / metabolism* Drosophila melanogaster / metabolism Embryo, Nonmammalian / diagnostic imaging Embryo, Nonmammalian / metabolism Endocytosis Exocytosis Fluorescent Dyes / chemistry Imaginal Discs / diagnostic imaging Imaginal Discs / metabolism Larva / metabolism Microscopy, Fluorescence / instrumentation Microscopy, Fluorescence / methods Models, Animal SNARE Proteins / chemistry SNARE Proteins / metabolism* Single Molecule Imaging / instrumentation Single Molecule Imaging / methods* |
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