| RRC ID |
57124
|
| Author |
Chai PC, Cruchet S, Wigger L, Benton R.
|
| Title |
Sensory neuron lineage mapping and manipulation in the Drosophila olfactory system.
|
| Journal |
Nat Commun
|
| Abstract |
Nervous systems exhibit myriad cell types, but understanding how this diversity arises is hampered by the difficulty to visualize and genetically-probe specific lineages, especially at early developmental stages prior to expression of unique molecular markers. Here, we use a genetic immortalization method to analyze the development of sensory neuron lineages in the Drosophila olfactory system, from their origin to terminal differentiation. We apply this approach to define a fate map of nearly all olfactory lineages and refine the model of temporal patterns of lineage divisions. Taking advantage of a selective marker for the lineage that gives rise to Or67d pheromone-sensing neurons and a genome-wide transcription factor RNAi screen, we identify the spatial and temporal requirements for Pointed, an ETS family member, in this developmental pathway. Transcriptomic analysis of wild-type and Pointed-depleted olfactory tissue reveals a universal requirement for this factor as a switch-like determinant of fates in these sensory lineages.
|
| Volume |
10(1)
|
| Pages |
643
|
| Published |
2019-2-7
|
| DOI |
10.1038/s41467-019-08345-4
|
| PII |
10.1038/s41467-019-08345-4
|
| PMID |
30733440
|
| PMC |
PMC6367400
|
| MeSH |
Animals
Drosophila
Drosophila Proteins / metabolism
Olfactory Bulb / cytology*
Olfactory Bulb / metabolism*
Sensory Receptor Cells / cytology*
Sensory Receptor Cells / metabolism*
|
| IF |
11.878
|
| Times Cited |
3
|
| Resource |
| Drosophila |
DGRC#105257
DGRC#104601
DGRC#108491
DGRC#108068 |
