論文 - 詳細
| RRC ID | 57135 |
|---|---|
| 著者 | Lee PT, Zirin J, Kanca O, Lin WW, Schulze KL, Li-Kroeger D, Tao R, Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, Housden BE, Mohr SE, Yamamoto S, Levis RW, Spradling AC, Perrimon N, Bellen HJ. |
| タイトル | A gene-specific T2A-GAL4 library for Drosophila. |
| ジャーナル | Elife |
| Abstract |
We generated a library of ~1000 Drosophila stocks in which we inserted a construct in the intron of genes allowing expression of GAL4 under control of endogenous promoters while arresting transcription with a polyadenylation signal 3' of the GAL4. This allows numerous applications. First, ~90% of insertions in essential genes cause a severe loss-of-function phenotype, an effective way to mutagenize genes. Interestingly, 12/14 chromosomes engineered through CRISPR do not carry second-site lethal mutations. Second, 26/36 (70%) of lethal insertions tested are rescued with a single UAS-cDNA construct. Third, loss-of-function phenotypes associated with many GAL4 insertions can be reverted by excision with UAS-flippase. Fourth, GAL4 driven UAS-GFP/RFP reports tissue and cell-type specificity of gene expression with high sensitivity. We report the expression of hundreds of genes not previously reported. Finally, inserted cassettes can be replaced with GFP or any DNA. These stocks comprise a powerful resource for assessing gene function. |
| 巻・号 | 7 |
| 公開日 | 2018-3-22 |
| DOI | 10.7554/eLife.35574 |
| PII | 35574 |
| PMID | 29565247 |
| PMC | PMC5898912 |
| MeSH | Animals Animals, Genetically Modified Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / cytology Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism Gene Expression Profiling Gene Library* Luminescent Proteins / genetics Luminescent Proteins / metabolism Mutagenesis, Insertional Organ Specificity / genetics Transcription Factors / genetics* Transcription Factors / metabolism |
| IF | 7.551 |
| 引用数 | 26 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 60 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | |