RRC ID 5714
Author Kamei Y, Itou J, Oda S, Masui M, Kim JH, Ishikawa T, Yuba S, Kinoshita M, Mitani H, Todo T.
Title Development of a convenient in vitro fertilization method using interspecific hybrids between Oryzias latipes and Oryzias curvinotus.
Journal Dev Growth Differ
Abstract Medaka is a small Asian freshwater teleost and has been an excellent model for fertilization studies for more than 50 years. Therefore, experimental procedures for in vitro fertilization (IVF) and cryopreservation of sperm are well established. In contrast, since the eggs or early embryos can not be cryopreserved, many females are killed to obtain unfertilized eggs for IVF. Recent progress in genomics is establishing medaka as a new model animal in functional genomics, and numerous mutant and transgenic strains have been established and stored as frozen sperm. Accumulated preserved resources require a simple and reliable recovery method for IVF. In this paper, we describe a method for obtaining a large number of unfertilized eggs without killing females, using sterile interspecific hybrids between Oryzias latipes and O. curvinotus. However, there is no report about the normality of offspring that were obtained by IVF using unfertilized eggs spawned in mating with the sterile hybrid male. In this paper, we have confirmed the reliability of the method regarding the influences on the next generation and also assessed conditions for efficient collection of unfertilized eggs. The method would be useful not only for fertilization studies but also for keeping transgenics and mutants, including a mutant library for a reverse genetic approach.
Volume 49(9)
Pages 721-30
Published 2007-12-1
DOI 10.1111/j.1440-169X.2007.00966.x
PMID 17983366
MeSH Animals Cryopreservation Female Fertilization in Vitro / methods* Hybridization, Genetic Male Oryzias / genetics* Oryzias / physiology Ovum Semen Preservation / methods
IF 1.638
Times Cited 1
Medaka OK-Cab (MT830) d-rR O. curvinotus (Hong Kong) (RS269) Tg(OlMA1-DsRed2) (TG930) Tg(OlMA1-GFP) (TG931)