RRC ID 57181
Author Xing G, Li M, Sun Y, Rui M, Zhuang Y, Lv H, Han J, Jia Z, Xie W.
Title Neurexin-Neuroligin 1 regulates synaptic morphology and functions via the WAVE regulatory complex in Drosophila neuromuscular junction.
Journal Elife
Abstract Neuroligins are postsynaptic adhesion molecules that are essential for postsynaptic specialization and synaptic function. But the underlying molecular mechanisms of neuroligin functions remain unclear. We found that Drosophila Neuroligin 1 (DNlg1) regulates synaptic structure and function through WAVE regulatory complex (WRC)-mediated postsynaptic actin reorganization. The disruption of DNlg1, DNlg2, or their presynaptic partner neurexin (DNrx) led to a dramatic decrease in the amount of F-actin. Further study showed that DNlg1, but not DNlg2 or DNlg3, directly interacts with the WRC via its C-terminal interacting receptor sequence. That interaction is required to recruit WRC to the postsynaptic membrane to promote F-actin assembly. Furthermore, the interaction between DNlg1 and the WRC is essential for DNlg1 to rescue the morphological and electrophysiological defects in dnlg1 mutants. Our results reveal a novel mechanism by which the DNrx-DNlg1 trans-synaptic interaction coordinates structural and functional properties at the neuromuscular junction.
Volume 7
Published 2018-3-14
DOI 10.7554/eLife.30457
PII 30457
PMID 29537369
PMC PMC5873926
MeSH Actins / chemistry Actins / genetics* Animals Cell Adhesion Molecules / genetics Cell Adhesion Molecules, Neuronal / chemistry Cell Adhesion Molecules, Neuronal / genetics* Drosophila melanogaster / genetics Drosophila melanogaster / physiology Glycoproteins / chemistry Glycoproteins / genetics* Membrane Proteins / chemistry Membrane Proteins / genetics Multiprotein Complexes / chemistry Multiprotein Complexes / genetics* Nerve Tissue Proteins / chemistry Nerve Tissue Proteins / genetics Neuromuscular Junction / genetics Neuromuscular Junction / physiology Neuropeptides / chemistry Neuropeptides / genetics* Presynaptic Terminals / chemistry Receptors, Glutamate / genetics Synapses / genetics Synaptic Transmission / genetics Wiskott-Aldrich Syndrome Protein Family / chemistry Wiskott-Aldrich Syndrome Protein Family / genetics
IF 7.551
Times Cited 9