論文 - 詳細
| RRC ID | 57181 |
|---|---|
| 著者 | Xing G, Li M, Sun Y, Rui M, Zhuang Y, Lv H, Han J, Jia Z, Xie W. |
| タイトル | Neurexin-Neuroligin 1 regulates synaptic morphology and functions via the WAVE regulatory complex in Drosophila neuromuscular junction. |
| ジャーナル | Elife |
| Abstract |
Neuroligins are postsynaptic adhesion molecules that are essential for postsynaptic specialization and synaptic function. But the underlying molecular mechanisms of neuroligin functions remain unclear. We found that Drosophila Neuroligin 1 (DNlg1) regulates synaptic structure and function through WAVE regulatory complex (WRC)-mediated postsynaptic actin reorganization. The disruption of DNlg1, DNlg2, or their presynaptic partner neurexin (DNrx) led to a dramatic decrease in the amount of F-actin. Further study showed that DNlg1, but not DNlg2 or DNlg3, directly interacts with the WRC via its C-terminal interacting receptor sequence. That interaction is required to recruit WRC to the postsynaptic membrane to promote F-actin assembly. Furthermore, the interaction between DNlg1 and the WRC is essential for DNlg1 to rescue the morphological and electrophysiological defects in dnlg1 mutants. Our results reveal a novel mechanism by which the DNrx-DNlg1 trans-synaptic interaction coordinates structural and functional properties at the neuromuscular junction. |
| 巻・号 | 7 |
| 公開日 | 2018-3-14 |
| DOI | 10.7554/eLife.30457 |
| PII | 30457 |
| PMID | 29537369 |
| PMC | PMC5873926 |
| MeSH | Actins / chemistry Actins / genetics* Animals Cell Adhesion Molecules / genetics Cell Adhesion Molecules, Neuronal / chemistry Cell Adhesion Molecules, Neuronal / genetics* Drosophila melanogaster / genetics Drosophila melanogaster / physiology Glycoproteins / chemistry Glycoproteins / genetics* Membrane Proteins / chemistry Membrane Proteins / genetics Multiprotein Complexes / chemistry Multiprotein Complexes / genetics* Nerve Tissue Proteins / chemistry Nerve Tissue Proteins / genetics Neuromuscular Junction / genetics Neuromuscular Junction / physiology Neuropeptides / chemistry Neuropeptides / genetics* Presynaptic Terminals / chemistry Receptors, Glutamate / genetics Synapses / genetics Synaptic Transmission / genetics Wiskott-Aldrich Syndrome Protein Family / chemistry Wiskott-Aldrich Syndrome Protein Family / genetics |
| IF | 7.551 |
| 引用数 | 9 |
| リソース情報 | |
| ショウジョウバエ | |