RRC ID |
57198
|
著者 |
Widmer YF, Bilican A, Bruggmann R, Sprecher SG.
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タイトル |
Regulators of Long-Term Memory Revealed by Mushroom Body-Specific Gene Expression Profiling in Drosophila melanogaster.
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ジャーナル |
Genetics
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Abstract |
Memory formation is achieved by genetically tightly controlled molecular pathways that result in a change of synaptic strength and synapse organization. While for short-term memory traces, rapidly acting biochemical pathways are in place, the formation of long-lasting memories requires changes in the transcriptional program of a cell. Although many genes involved in learning and memory formation have been identified, little is known about the genetic mechanisms required for changing the transcriptional program during different phases of long-term memory (LTM) formation. With Drosophila melanogaster as a model system, we profiled transcriptomic changes in the mushroom body-a memory center in the fly brain-at distinct time intervals during appetitive olfactory LTM formation using the targeted DamID technique. We describe the gene expression profiles during these phases and tested 33 selected candidate genes for deficits in LTM formation using RNAi knockdown. We identified 10 genes that enhance or decrease memory when knocked-down in the mushroom body. For vajk-1 and hacd1-the two strongest hits-we gained further support for their crucial role in appetitive learning and forgetting. These findings show that profiling gene expression changes in specific cell-types harboring memory traces provides a powerful entry point to identify new genes involved in learning and memory. The presented transcriptomic data may further be used as resource to study genes acting at different memory phases.
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巻・号 |
209(4)
|
ページ |
1167-1181
|
公開日 |
2018-8-1
|
DOI |
10.1534/genetics.118.301106
|
PII |
genetics.118.301106
|
PMID |
29925565
|
PMC |
PMC6063240
|
MeSH |
Animals
Animals, Genetically Modified / growth & development
Behavior, Animal
Drosophila Proteins / genetics*
Drosophila melanogaster / genetics
Drosophila melanogaster / growth & development*
Enoyl-CoA Hydratase / genetics*
Gene Expression Profiling / methods*
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Memory, Long-Term*
Mushroom Bodies / chemistry*
Organ Specificity
RNA Interference
|
IF |
3.564
|
引用数 |
8
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リソース情報 |
ショウジョウバエ |
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