論文 - 詳細
| RRC ID | 57200 |
|---|---|
| 著者 | Duan R, Kim JH, Shilagardi K, Schiffhauer ES, Lee DM, Son S, Li S, Thomas C, Luo T, Fletcher DA, Robinson DN, Chen EH. |
| タイトル | Spectrin is a mechanoresponsive protein shaping fusogenic synapse architecture during myoblast fusion. |
| ジャーナル | Nat Cell Biol |
| Abstract |
Spectrin is a membrane skeletal protein best known for its structural role in maintaining cell shape and protecting cells from mechanical damage. Here, we report that α/βH-spectrin (βH is also called karst) dynamically accumulates and dissolves at the fusogenic synapse between fusing Drosophila muscle cells, where an attacking fusion partner invades its receiving partner with actin-propelled protrusions to promote cell fusion. Using genetics, cell biology, biophysics and mathematical modelling, we demonstrate that spectrin exhibits a mechanosensitive accumulation in response to shear deformation, which is highly elevated at the fusogenic synapse. The transiently accumulated spectrin network functions as a cellular fence to restrict the diffusion of cell-adhesion molecules and a cellular sieve to constrict the invasive protrusions, thereby increasing the mechanical tension of the fusogenic synapse to promote cell membrane fusion. Our study reveals a function of spectrin as a mechanoresponsive protein and has general implications for understanding spectrin function in dynamic cellular processes. |
| 巻・号 | 20(6) |
| ページ | 688-698 |
| 公開日 | 2018-6-1 |
| DOI | 10.1038/s41556-018-0106-3 |
| PII | 10.1038/s41556-018-0106-3 |
| PMID | 29802406 |
| PMC | PMC6397639 |
| MeSH | Animals Animals, Genetically Modified Cell Fusion* Cell Line Cell Membrane / metabolism* Cell Membrane / ultrastructure Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Drosophila melanogaster / ultrastructure Mechanotransduction, Cellular* Membrane Fusion* Mice Microscopy, Fluorescence Models, Biological Myoblasts / metabolism* Myoblasts / ultrastructure Myoblasts, Skeletal / metabolism Spectrin / genetics Spectrin / metabolism* Stress, Mechanical Time Factors |
| IF | 17.728 |
| 引用数 | 10 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 37 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | |