| RRC ID |
5721
|
| 著者 |
Fukuoka T, Morita T, Konishi M, Imura T, Sakai H, Kitamoto D.
|
| タイトル |
Structural characterization and surface-active properties of a new glycolipid biosurfactant, mono-acylated mannosylerythritol lipid, produced from glucose by Pseudozyma antarctica.
|
| ジャーナル |
Appl Microbiol Biotechnol
|
| Abstract |
Mannosylerythritol lipids (MELs), which are glycolipid biosurfactants produced by Pseudozyma yeasts, show not only excellent interfacial properties but also versatile biochemical actions. In the course of MEL production from glucose as the sole carbon source, P. antarctica was found to produce unknown glycolipids more hydrophilic than conventional "di-acylated MELs," which have two fatty acyl esters on the mannose moiety. Based on a detailed characterization, the most hydrophilic one was identified as 4-O-(3'-O-alka(e)noyl-beta-D: -mannopyranosyl)-D: -erythritol namely, "mono-acylated MEL." The mono-acylated MEL reduced the surface tension of water to 33.8 mN/m at a critical micelle concentration (CMC) of 3.6 x 10(-4) M, and its hydrophilic-lipophilic balance was tentatively calculated to be 12.15. The observed CMC was 100-fold higher than that of the MELs hitherto reported. Interestingly, of the yeast strains of the genus Pseudozyma, only P. antarctica and P. parantarctica gave the mono-acylated MEL from glucose, despite a great diversity of di-acylated MEL producers in the genus. These strains produced MELs including the mono-acylated one at a rate of 20-25%. From these results, the new MEL is likely to have great potential for use in oil-in-water-type emulsifiers and washing detergents because of its higher water solubility compared to conventional MELs and will thus contribute to facilitating a broad range of applications for the environmentally advanced surfactants.
|
| 巻・号 |
76(4)
|
| ページ |
801-10
|
| 公開日 |
2007-9-1
|
| DOI |
10.1007/s00253-007-1051-4
|
| PMID |
17607573
|
| MeSH |
Anti-Infective Agents / chemistry
Anti-Infective Agents / metabolism
Anti-Infective Agents / pharmacology
Basidiomycota / metabolism*
Biotechnology / methods*
Chromatography, High Pressure Liquid
Glucose / metabolism*
Glycolipids / chemistry*
Glycolipids / metabolism*
Glycolipids / pharmacology
Hydrophobic and Hydrophilic Interactions
Microbial Sensitivity Tests
Nuclear Magnetic Resonance, Biomolecular
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Surface Tension
Surface-Active Agents / chemistry*
Surface-Active Agents / metabolism*
Surface-Active Agents / pharmacology
|
| IF |
3.53
|
| 引用数 |
50
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM10317
JCM3941
JCM10318
JCM10323
JCM3931
JCM11752
JCM10324 |
