RRC ID 57265
著者 Zhu L, Kandasamy SK, Liao SE, Fukunaga R.
タイトル LOTUS domain protein MARF1 binds CCR4-NOT deadenylase complex to post-transcriptionally regulate gene expression in oocytes.
ジャーナル Nat Commun
Abstract Post-transcriptional regulation of gene expression plays an essential role during oocyte maturation. Here we report that Drosophila MARF1 (Meiosis Regulator And mRNA Stability Factor 1), which consists of one RNA-recognition motif and six tandem LOTUS domains with unknown molecular function, is essential for oocyte maturation. When tethered to a reporter mRNA, MARF1 post-transcriptionally silences reporter expression by shortening reporter mRNA poly-A tail length and thereby reducing reporter protein level. This activity is mediated by the MARF1 LOTUS domain, which binds the CCR4-NOT deadenylase complex. MARF1 binds cyclin A mRNA and shortens its poly-A tail to reduce Cyclin A protein level during oocyte maturation. This study identifies MARF1 as a regulator in oocyte maturation and defines the conserved LOTUS domain as a post-transcriptional effector domain that recruits CCR4-NOT deadenylase complex to shorten target mRNA poly-A tails and suppress their translation.
巻・号 9(1)
ページ 4031
公開日 2018-10-2
DOI 10.1038/s41467-018-06404-w
PII 10.1038/s41467-018-06404-w
PMID 30279526
PMC PMC6168497
MeSH Animals Animals, Genetically Modified Cyclin A / metabolism* Drosophila Drosophila Proteins / genetics Drosophila Proteins / metabolism* Female Fertility Male Meiosis Membrane Proteins / genetics Membrane Proteins / metabolism* Oocytes / metabolism* Protein Transport RNA 3' End Processing* Ribonucleases / metabolism*
IF 11.878
引用数 8
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