RRC ID 57272
Author Mohammad F, Stewart JC, Ott S, Chlebikova K, Chua JY, Koh TW, Ho J, Claridge-Chang A.
Title Optogenetic inhibition of behavior with anion channelrhodopsins.
Journal Nat Methods
Abstract Optogenetics uses light exposure to manipulate physiology in genetically modified organisms. Abundant tools for optogenetic excitation are available, but the limitations of current optogenetic inhibitors present an obstacle to demonstrating the necessity of neuronal circuits. Here we show that anion channelrhodopsins can be used to specifically and rapidly inhibit neural systems involved in Drosophila locomotion, wing expansion, memory retrieval and gustation, thus demonstrating their broad utility in the circuit analysis of behavior.
Volume 14(3)
Pages 271-274
Published 2017-3
DOI 10.1038/nmeth.4148
PII nmeth.4148
PMID 28114289
MeSH Action Potentials / physiology Animals Behavior, Animal / drug effects* Behavior, Animal / physiology Drosophila / physiology* Light Locomotion / physiology Neural Pathways / physiology* Neurons / physiology Optogenetics / methods* Organisms, Genetically Modified Rhodopsin / pharmacology* Taste Perception / physiology Voltage-Dependent Anion Channels / physiology
IF 28.467
Times Cited 50