RRC ID 57288
Author Tokusumi T, Tokusumi Y, Schulz RA.
Title The mir-7 and bag of marbles genes regulate Hedgehog pathway signaling in blood cell progenitors in Drosophila larval lymph glands.
Journal Genesis
Abstract Hedgehog (Hh) pathway signaling is crucial for the maintenance of blood cell progenitors in the lymph gland hematopoietic organ present in Drosophila third instar larvae. Previous studies from our lab have likewise shown the importance of the mir-7 and bag of marbles (bam) genes in maintaining the progenitor state. Thus, we sought to investigate a possible interaction between the Hh pathway and mir-7/bam in the prohemocyte population within this hematopoietic tissue. Gain of function mir-7 was able to rescue a blood cell progenitor depletion phenotype caused by Patched (Ptc) inhibition of Hh pathway signaling in these cells. Similarly, expression of a dominant/negative version of Ptc was able to rescue the severe reduction of prohemocytes due to bam loss of function. Furthermore, we demonstrated that Suppressor of fused [Su(fu)], another known inhibitor of Hh signaling, likely serves as a translational repression target of the mir-7 miRNA. Our results suggest the mir-7/bam combination regulates the Hh signaling network through repression of Su(fu) to maintain hemocyte progenitors in the larval lymph gland.
Volume 56(5)
Pages e23210
Published 2018-5-1
DOI 10.1002/dvg.23210
PMID 29663653
PMC PMC5980778
MeSH Animals Blood Cells Cell Differentiation / genetics Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Gene Expression Regulation, Developmental / genetics Hedgehog Proteins / genetics Hematopoietic Stem Cells / metabolism Larva / metabolism Lymph Nodes / embryology Lymph Nodes / metabolism MicroRNAs / genetics MicroRNAs / metabolism* Receptors, Immunologic / metabolism Repressor Proteins / genetics Signal Transduction
IF 1.74
Times Cited 3
Drosophila DGRC#105442