論文 - 詳細
| RRC ID | 5731 |
|---|---|
| 著者 | Nunoura T, Oida H, Miyazaki J, Miyashita A, Imachi H, Takai K. |
| タイトル | Quantification of mcrA by fluorescent PCR in methanogenic and methanotrophic microbial communities. |
| ジャーナル | FEMS Microbiol Ecol |
| Abstract |
A quantitative fluorogenic PCR method for detecting methanogenic and methanotrophic orders was established using a refined primer set for the methyl coenzyme M reductase subunit A gene (mcrA). The method developed was applied to several microbial communities in which diversity and abundance of methanogens or anaerobic methanotrophs (ANMEs) was identified by 16S rRNA gene clone analysis, and strong correlations between the copy numbers of mcrA with those of archaeal 16S rRNA genes in the communities were observed. The assay can be applied to detecting and assessing the abundance of methanogens and/or ANMEs in anoxic environments that could not be detected by 16S rRNA gene sequence analyses. |
| 巻・号 | 64(2) |
| ページ | 240-7 |
| 公開日 | 2008-5-1 |
| DOI | 10.1111/j.1574-6941.2008.00451.x |
| PII | FEM451 |
| PMID | 18318714 |
| MeSH | Archaea / enzymology* Archaeal Proteins / genetics DNA Primers / genetics DNA, Archaeal / genetics DNA, Ribosomal / genetics Fluorescence Gene Dosage* Methane / metabolism* Oxidoreductases / genetics* Polymerase Chain Reaction / methods* RNA, Ribosomal, 16S / genetics |
| IF | 3.675 |
| 引用数 | 69 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM10825 JCM13029 JCM10723 JCM10045 JCM10549 JCM11834 JCM9639 |