Reference - Detail
| RRC ID | 57356 |
|---|---|
| Author | Li S, Koe CT, Tay ST, Tan ALK, Zhang S, Zhang Y, Tan P, Sung WK, Wang H. |
| Title | An intrinsic mechanism controls reactivation of neural stem cells by spindle matrix proteins. |
| Journal | Nat Commun |
| Abstract |
The switch between quiescence and proliferation is central for neurogenesis and its alteration is linked to neurodevelopmental disorders such as microcephaly. However, intrinsic mechanisms that reactivate Drosophila larval neural stem cells (NSCs) to exit from quiescence are not well established. Here we show that the spindle matrix complex containing Chromator (Chro) functions as a key intrinsic regulator of NSC reactivation downstream of extrinsic insulin/insulin-like growth factor signalling. Chro also prevents NSCs from re-entering quiescence at later stages. NSC-specific in vivo profiling has identified many downstream targets of Chro, including a temporal transcription factor Grainy head (Grh) and a neural stem cell quiescence-inducing factor Prospero (Pros). We show that spindle matrix proteins promote the expression of Grh and repress that of Pros in NSCs to govern their reactivation. Our data demonstrate that nuclear Chro critically regulates gene expression in NSCs at the transition from quiescence to proliferation.The spindle matrix proteins, including Chro, are known to regulate mitotic spindle assembly in the cytoplasm. Here the authors show that in Drosophila larval brain, Chro promotes neural stem cell (NSC) reactivation and prevents activated NSCs from entering quiescence, and that Chro carries out such a role by regulating the expression of key transcription factors in the nucleus. |
| Volume | 8(1) |
| Pages | 122 |
| Published | 2017-7-25 |
| DOI | 10.1038/s41467-017-00172-9 |
| PII | 10.1038/s41467-017-00172-9 |
| PMID | 28744001 |
| PMC | PMC5526931 |
| MeSH | Animals Animals, Genetically Modified Blotting, Western Cell Cycle Proteins DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / cytology Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Gene Expression Profiling / methods Larva / cytology Larva / genetics Larva / metabolism Microscopy, Confocal Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neural Stem Cells / metabolism* Nuclear Matrix-Associated Proteins / genetics* Nuclear Matrix-Associated Proteins / metabolism Nuclear Proteins / genetics Nuclear Proteins / metabolism Phosphoproteins / genetics* Phosphoproteins / metabolism RNA Interference Transcription Factors / genetics Transcription Factors / metabolism |
| IF | 11.878 |
| Times Cited | 8 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 16 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | DGRC#111163 |