RRC ID |
57375
|
Author |
Sereti KI, Oikonomopoulos A, Unno K, Liao R.
|
Title |
Methods to study the proliferation and differentiation of cardiac side population (CSP) cells.
|
Journal |
Methods Mol Biol
|
Abstract |
Investigation of cardiac progenitor cell proliferation and differentiation is essential for both the basic understanding of progenitor cell biology as well as the development of cellular therapeutics for tissue regeneration. Herein, we describe techniques used for the analysis of CSP cell proliferation, cell cycle status, and cardiomyogenic differentiation.
|
Volume |
1036
|
Pages |
95-106
|
Published |
2013-1-1
|
DOI |
10.1007/978-1-62703-511-8_9
|
PMID |
23807790
|
MeSH |
Animals
Animals, Newborn
Cell Cycle
Cell Differentiation*
Cell Proliferation*
Cell Separation / methods*
Cells, Cultured
Flow Cytometry
Myocytes, Cardiac / cytology*
Myocytes, Cardiac / metabolism
Rats
Side-Population Cells / cytology*
Side-Population Cells / metabolism
Stem Cells / cytology*
Stem Cells / metabolism
|
Resource |
DNA material |
mKO2-hCdt1(30/120) / pCSII-EF (RDB15267)
mAG-hGeminin(1/110) / pCSII-EF (RDB15268)
pCAG-HIVgp (RDB04394)
pCMV-VSV-G-RSV-Rev (RDB04393) |