RRC ID 57389
Author Maclean HJ, Kristensen TN, Sørensen JG, Overgaard J.
Title Laboratory maintenance does not alter ecological and physiological patterns among species: a Drosophila case study.
Journal J Evol Biol
Abstract Large comparative studies in animal ecology, physiology and evolution often use animals reared in the laboratory for many generations; however, the relevance of these studies hinges on the assumption that laboratory populations are still representative for their wild living conspecifics. In this study, we investigate whether laboratory-maintained and freshly collected animal populations are fundamentally different and whether data from laboratory-maintained animals are valid to use in large comparative investigations of ecological and physiological patterns. Here, we obtained nine species of Drosophila with paired populations of laboratory-maintained and freshly collected flies. These species, representing a range of ecotypes, were assayed for four stress-tolerance, two body-size traits and six life-history traits. For all of these traits, we observed small differences in species-specific comparisons between field and laboratory populations; however, these differences were unsystematic and laboratory maintenance did not eclipse fundamental species characteristics. To investigate whether laboratory maintenance influence the general patterns in comparative studies, we correlated stress tolerance and life-history traits with environmental traits for the laboratory-maintained and freshly collected populations. Based on this analysis, we found that the comparative physiological and ecological trait correlations are similar irrespective of provenience. This finding is important for comparative biology in general because it validates comparative meta-analyses based on laboratory-maintained populations.
Volume 31(4)
Pages 530-542
Published 2018-4
DOI 10.1111/jeb.13241
PMID 29446196
MeSH Animals Body Composition Culture Techniques* Drosophila / physiology* Female Life History Traits Stress, Physiological
IF 2.541
Times Cited 14