論文 - 詳細
| RRC ID | 57430 |
|---|---|
| 著者 | Kumada K, Fuse N, Tamura T, Okamori C, Kurata S. |
| タイトル | HSP70/DNAJA3 chaperone/cochaperone regulates NF-κB activity in immune responses. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Nuclear factor kappa B (NF-κB) controls the transcription of various genes in response to immune stimuli. Our previous study revealed that the Droj2/DNAJA3 cochaperone contributes to the NF-κB pathway in Drosophila and humans. In general, the cochaperone is associated with the 70-kDa heat shock protein (HSP70) chaperone and the complex supports the folding of diverse target proteins. The cochaperone/chaperone functions in the NF-κB pathway, however, are not clearly understood. Here, we report that HSP70 proteins are involved in activating canonical NF-κB signaling during immune responses. In human cultured cells, HSP70 inhibitor destabilized the IKKβ/IκBα/NF-κB p65 complex and dampened the phosphorylation of NF-κB p65 in response to flagellin stimulation. We identified HSPA1A and HSPA8 as the HSP70 family proteins that physically interact with DNAJA3, and established their requirement for the phosphorylation of NF-κB p65. Furthermore, as in flies with knockdown of Droj2, flies with knockdown of Hsc70-4, a Drosophila homolog of HSPA8, were more susceptible to infection. Our results suggest that the chaperone/cochaperone complex regulates NF-κB immune signaling in an evolutionarily conserved manner. |
| 巻・号 | 513(4) |
| ページ | 947-951 |
| 公開日 | 2019-6-11 |
| DOI | 10.1016/j.bbrc.2019.04.077 |
| PII | S0006-291X(19)30711-9 |
| PMID | 31005254 |
| MeSH | Animals Cells, Cultured Drosophila HSC70 Heat-Shock Proteins / metabolism HSP40 Heat-Shock Proteins / metabolism HSP40 Heat-Shock Proteins / physiology* HSP70 Heat-Shock Proteins / metabolism HSP70 Heat-Shock Proteins / physiology* Humans Immunity* Molecular Chaperones / immunology Molecular Chaperones / physiology* NF-kappa B / immunology NF-kappa B / metabolism* Phosphorylation |
| IF | 2.705 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 4264R-1 |