RRC ID 57511
Author Hokamura A, Fujino K, Isoda Y, Arizono K, Shiratsuchi H, Matsusaki H.
Title Characterization and identification of the proteins bound to two types of polyhydroxyalkanoate granules in Pseudomonas sp. 61-3.
Journal Biosci Biotechnol Biochem
Abstract Pseudomonas sp. 61-3 accumulates two types of polyhydroxyalkanoates (PHAs), poly(3-hydroxybutyrate) [P(3HB)], and poly(3HB-co-3-hydroxyalkanoates) [P(3HB-co-3HA)], and some proteins associated with their PHA granules have been identified. To date, PhaFPs (GA36) and PhaIPs (GA18) were identified from P(3HB-co-3HA) granules. In this study, the gene encoding GA24 associated with P(3HB) granule was identified as phbPPs. PhbPPs was composed of 192 amino acids with a calculated molecular mass of 20.4 kDa and was assumed to be a phasin. phbFPs gene and unknown ORF were also found on phb locus. PhbFPs was anticipated to be the transcriptional repressor of phbPPs gene. PhbPPs was bound to the P(3HB-co-3HA) granules with 3HB composition of more than 87 mol%, and PhaIPs and PhaFPs were bound to the P(3HB-co-3HA) granules with 3HA (C6-C12) composition of more than 13 mol% in the producing cells, suggesting that localization of these proteins is attributed to the monomer compositions of the copolymers.
Volume 79(8)
Pages 1369-77
Published 2015-1-1
DOI 10.1080/09168451.2015.1023250
PMID 25971301
MeSH Bacterial Proteins / chemistry Bacterial Proteins / isolation & purification Bacterial Proteins / metabolism* Hydroxybutyrates / chemistry Hydroxybutyrates / metabolism* Inclusion Bodies / chemistry Inclusion Bodies / metabolism Molecular Weight Polyesters / chemistry Polyesters / metabolism* Polyhydroxyalkanoates / chemistry Polyhydroxyalkanoates / metabolism* Pseudomonas / metabolism* Pseudomonas / pathogenicity
IF 1.297
Times Cited 5
General Microbes JCM 10015