| RRC ID |
57511
|
| 著者 |
Hokamura A, Fujino K, Isoda Y, Arizono K, Shiratsuchi H, Matsusaki H.
|
| タイトル |
Characterization and identification of the proteins bound to two types of polyhydroxyalkanoate granules in Pseudomonas sp. 61-3.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
Pseudomonas sp. 61-3 accumulates two types of polyhydroxyalkanoates (PHAs), poly(3-hydroxybutyrate) [P(3HB)], and poly(3HB-co-3-hydroxyalkanoates) [P(3HB-co-3HA)], and some proteins associated with their PHA granules have been identified. To date, PhaFPs (GA36) and PhaIPs (GA18) were identified from P(3HB-co-3HA) granules. In this study, the gene encoding GA24 associated with P(3HB) granule was identified as phbPPs. PhbPPs was composed of 192 amino acids with a calculated molecular mass of 20.4 kDa and was assumed to be a phasin. phbFPs gene and unknown ORF were also found on phb locus. PhbFPs was anticipated to be the transcriptional repressor of phbPPs gene. PhbPPs was bound to the P(3HB-co-3HA) granules with 3HB composition of more than 87 mol%, and PhaIPs and PhaFPs were bound to the P(3HB-co-3HA) granules with 3HA (C6-C12) composition of more than 13 mol% in the producing cells, suggesting that localization of these proteins is attributed to the monomer compositions of the copolymers.
|
| 巻・号 |
79(8)
|
| ページ |
1369-77
|
| 公開日 |
2015-1-1
|
| DOI |
10.1080/09168451.2015.1023250
|
| PMID |
25971301
|
| MeSH |
Bacterial Proteins / chemistry
Bacterial Proteins / isolation & purification
Bacterial Proteins / metabolism*
Hydroxybutyrates / chemistry
Hydroxybutyrates / metabolism*
Inclusion Bodies / chemistry
Inclusion Bodies / metabolism
Molecular Weight
Polyesters / chemistry
Polyesters / metabolism*
Polyhydroxyalkanoates / chemistry
Polyhydroxyalkanoates / metabolism*
Pseudomonas / metabolism*
Pseudomonas / pathogenicity
|
| IF |
1.297
|
| 引用数 |
5
|
| リソース情報 |
| 一般微生物 |
JCM 10015 |
