論文 - 詳細
| RRC ID | 57511 |
|---|---|
| 著者 | Hokamura A, Fujino K, Isoda Y, Arizono K, Shiratsuchi H, Matsusaki H. |
| タイトル | Characterization and identification of the proteins bound to two types of polyhydroxyalkanoate granules in Pseudomonas sp. 61-3. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Pseudomonas sp. 61-3 accumulates two types of polyhydroxyalkanoates (PHAs), poly(3-hydroxybutyrate) [P(3HB)], and poly(3HB-co-3-hydroxyalkanoates) [P(3HB-co-3HA)], and some proteins associated with their PHA granules have been identified. To date, PhaFPs (GA36) and PhaIPs (GA18) were identified from P(3HB-co-3HA) granules. In this study, the gene encoding GA24 associated with P(3HB) granule was identified as phbPPs. PhbPPs was composed of 192 amino acids with a calculated molecular mass of 20.4 kDa and was assumed to be a phasin. phbFPs gene and unknown ORF were also found on phb locus. PhbFPs was anticipated to be the transcriptional repressor of phbPPs gene. PhbPPs was bound to the P(3HB-co-3HA) granules with 3HB composition of more than 87 mol%, and PhaIPs and PhaFPs were bound to the P(3HB-co-3HA) granules with 3HA (C6-C12) composition of more than 13 mol% in the producing cells, suggesting that localization of these proteins is attributed to the monomer compositions of the copolymers. |
| 巻・号 | 79(8) |
| ページ | 1369-77 |
| 公開日 | 2015-1-1 |
| DOI | 10.1080/09168451.2015.1023250 |
| PMID | 25971301 |
| MeSH | Bacterial Proteins / chemistry Bacterial Proteins / isolation & purification Bacterial Proteins / metabolism* Hydroxybutyrates / chemistry Hydroxybutyrates / metabolism* Inclusion Bodies / chemistry Inclusion Bodies / metabolism Molecular Weight Polyesters / chemistry Polyesters / metabolism* Polyhydroxyalkanoates / chemistry Polyhydroxyalkanoates / metabolism* Pseudomonas / metabolism* Pseudomonas / pathogenicity |
| IF | 1.297 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 10015 |