RRC ID 57553
Author Yoshitomi T, Shimada N, Iijima K, Hashizume M, Yoshimoto K.
Title Polyethyleneimine-induced astaxanthin accumulation in the green alga Haematococcus pluvialis by increased oxidative stress.
Journal J Biosci Bioeng
Abstract The unicellular green microalga Haematococcus pluvialis accumulates large amounts of the red ketocarotenoid astaxanthin under stress conditions such as nitrogen deficiency. In this study, we discovered an astaxanthin accumulation in H. pluvialis cells by the addition of a synthetic cationic polymer, polyethyleneimine (PEI), into the cell culture. With an increase in PEI amount, amount of astaxanthin accumulation was increased. To investigate the mechanism for the accumulation of astaxanthin by the addition of PEI in H. pluvialis cells, we measured a localization of PEI in the cells and a production of reactive oxygen species. PEI was internalized in the cells through the negatively-charged cell walls, leading to excessive production of reactive oxygen species in the cells. Thus, the increased oxidative stress by cellular uptake of PEI resulted in the acceleration of astaxanthin accumulation in H. pluvialis.
Volume 128(6)
Pages 751-754
Published 2019-12-1
DOI 10.1016/j.jbiosc.2019.06.002
PII S1389-1723(19)30310-X
PMID 31253510
MeSH Chlorophyta / drug effects* Chlorophyta / metabolism Color Oxidative Stress / drug effects* Polyethyleneimine / chemistry Polyethyleneimine / pharmacology* Reactive Oxygen Species / metabolism Xanthophylls / metabolism
IF 2.032
Times Cited 0
Algae NIES-144