| RRC ID |
5758
|
| 著者 |
Allen MA, Goh F, Leuko S, Echigo A, Mizuki T, Usami R, Kamekura M, Neilan BA, Burns BP.
|
| タイトル |
Haloferax elongans sp. nov. and Haloferax mucosum sp. nov., isolated from microbial mats from Hamelin Pool, Shark Bay, Australia.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
Extremely halophilic archaea were cultivated from smooth and pustular microbial mats collected from Hamelin Pool, Shark Bay, Western Australia. On the basis of morphology, two phenotypes were present and 16S rRNA gene sequence analysis indicated that all strains were most closely related to members of the genus Haloferax (98.1-99.4 % similarity). One representative strain from each phenotype was selected for further taxonomic characterization. Strain SA5T, isolated from the smooth mat, formed small ( approximately 1 mm diameter), red, translucent colonies on agar medium and strain PA12T, isolated from the pustular mat, formed large (3-5 mm diameter), pink, mucoid, domed colonies. Both strains grew in media with 1.7-5.1 M NaCl, required at least 0.2 M Mg2+ for growth and had pH optima of 7.4. The 16S rRNA gene similarity between strains SA5T and PA12T was 97.1 %. Physiological properties, G+C content and polar lipid composition supported placement of both strains in the genus Haloferax. Phenotypic analysis indicated that the two strains were distinct from each other and from all other members of the genus. This was confirmed by the low DNA-DNA relatedness between strains SA5T and PA12T (18-30 %) and between both strains and all other recognized Haloferax species. Two novel species of the genus Haloferax are proposed to accommodate these novel isolates, Haloferax elongans sp. nov. (type strain SA5T=JCM 14791T=ATCC BAA-1513T=UNSW 104100T) and Haloferax mucosum sp. nov. (type strain PA12T=JCM 14792T=ATCC BAA-1512T=UNSW 104200T).
|
| 巻・号 |
58(Pt 4)
|
| ページ |
798-802
|
| 公開日 |
2008-4-1
|
| DOI |
10.1099/ijs.0.65360-0
|
| PII |
58/4/798
|
| PMID |
18398172
|
| MeSH |
Base Composition
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Genes, Bacterial
Haloferax / classification*
Haloferax / genetics
Haloferax / isolation & purification*
Haloferax / metabolism
Lipids / analysis
Molecular Sequence Data
Phenotype
Phylogeny
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Seawater / microbiology
Sequence Homology, Nucleic Acid
Species Specificity
Western Australia
|
| IF |
2.415
|
| 引用数 |
27
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM8866
JCM8879
JCM9276
JCM8864
JCM8863
JCM10717
JCM12327
JCM13924
JCM13917
JCM14791
JCM14792 |
