Reference - Detail
| RRC ID | 57594 |
|---|---|
| Author | Lee CW, Kwon YC, Lee Y, Park MY, Choe KM. |
| Title | cdc37 is essential for JNK pathway activation and wound closure in Drosophila. |
| Journal | Mol Biol Cell |
| Abstract |
Wound closure in the Drosophila larval epidermis mainly involves nonproliferative, endocyling epithelial cells. Consequently, it is largely mediated by cell growth and migration. We discovered that both cell growth and migration in Drosophila require the cochaperone-encoding gene cdc37. Larvae lacking cdc37 in the epidermis failed to close wounds, and the cells of the epidermis failed to change cell shape and polarize. Likewise, wound-induced cell growth was significantly reduced, and correlated with a reduction in the size of the cell nucleus. The c-Jun N-terminal kinase (JNK) pathway, which is essential for wound closure, was not typically activated in injured cdc37 knockdown larvae. In addition, JNK, Hep, Mkk4, and Tak1 protein levels were reduced, consistent with previous reports showing that Cdc37 is important for the stability of various client kinases. Protein levels of the integrin β subunit and its wound-induced protein expression were also reduced, reflecting the disruption of JNK activation, which is crucial for expression of integrin β during wound closure. These results are consistent with a role of Cdc37 in maintaining the stability of the JNK pathway kinases, thus mediating cell growth and migration during Drosophila wound healing. |
| Volume | 30(21) |
| Pages | 2651-2658 |
| Published | 2019-10-1 |
| DOI | 10.1091/mbc.E18-12-0822 |
| PMID | 31483695 |
| PMC | PMC6761768 |
| MeSH | Animals Animals, Genetically Modified Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Movement / genetics Cell Proliferation / genetics Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Epidermis / injuries Epidermis / metabolism JNK Mitogen-Activated Protein Kinases / genetics JNK Mitogen-Activated Protein Kinases / metabolism Larva / cytology Larva / genetics Larva / metabolism MAP Kinase Kinase Kinases / genetics MAP Kinase Kinase Kinases / metabolism MAP Kinase Signaling System* Molecular Chaperones / genetics Molecular Chaperones / metabolism* Protein Stability RNA Interference Wound Healing / genetics Wound Healing / physiology* |
| IF | 3.905 |
| Times Cited | 1 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | 12019R-2 |