RRC ID 57617
Author Houtz P, Bonfini A, Bing X, Buchon N.
Title Recruitment of Adult Precursor Cells Underlies Limited Repair of the Infected Larval Midgut in Drosophila.
Journal Cell Host Microbe
Abstract Surviving infection requires immune and repair mechanisms. Developing organisms face the additional challenge of integrating these mechanisms with tightly controlled developmental processes. The larval Drosophila midgut lacks dedicated intestinal stem cells. We show that, upon infection, larvae perform limited repair using adult midgut precursors (AMPs). AMPs differentiate in response to damage to generate new enterocytes, transiently depleting their pool. Developmental delay allows for AMP reconstitution, ensuring the completion of metamorphosis. Notch signaling is required for the differentiation of AMPs into the encasing, niche-like peripheral cells (PCs), but not to differentiate PCs into enterocytes. Dpp (TGF-β) signaling is sufficient, but not necessary, to induce PC differentiation into enterocytes. Infection-induced JAK-STAT pathway is both required and sufficient for differentiation of AMPs and PCs into new enterocytes. Altogether, this work highlights the constraints imposed by development on an organism's response to infection and demonstrates the transient use of adult precursors for tissue repair.
Volume 26(3)
Pages 412-425.e5
Published 2019-9-11
DOI 10.1016/j.chom.2019.08.006
PII S1931-3128(19)30409-3
PMID 31492656
MeSH Animals Cell Differentiation Disease Models, Animal Drosophila / growth & development* Drosophila / metabolism* Drosophila / microbiology Drosophila / physiology Drosophila Proteins / genetics Drosophila Proteins / metabolism* Enterocytes / metabolism Gastrointestinal Tract / immunology Gastrointestinal Tract / metabolism* Gastrointestinal Tract / microbiology Gastrointestinal Tract / pathology Gram-Negative Bacterial Infections / metabolism Gram-Negative Bacterial Infections / pathology Janus Kinases / metabolism Larva / immunology Larva / metabolism* Larva / microbiology Metamorphosis, Biological Pectobacterium carotovorum / pathogenicity STAT Transcription Factors / metabolism Signal Transduction / physiology Stem Cells / metabolism Transcription Factors / metabolism Transcriptome
IF 15.753
Times Cited 2