論文 - 詳細
| RRC ID | 57650 |
|---|---|
| 著者 | Kawamura F, Inaki M, Katafuchi A, Abe Y, Tsuyama N, Kurosu Y, Yanagi A, Higuchi M, Muto S, Yamaura T, Suzuki H, Noji H, Suzuki S, Yoshida MA, Sasatani M, Kamiya K, Onodera M, Sakai A. |
| タイトル | Establishment of induced pluripotent stem cells from normal B cells and inducing AID expression in their differentiation into hematopoietic progenitor cells. |
| ジャーナル | Sci Rep |
| Abstract |
B cell derived induced pluripotent stem cells (BiPSCs) were recently established from peripheral blood B cells by the simultaneous transfection of Yamanaka factors (Oct3/4, Sox2, Klf4, c-Myc) and C/EBPα using a Sendai virus vector. Here, using a different method, we established BiPSCs with immunoglobulin heavy chain (IgH) gene rearrangement from normal B cells purified from lymph nodes. The critical points of our method are pre-stimulation of B cells with IL-21 and CD40-ligand (CD40L), followed by consecutive transfection of highly concentrated Yamanaka factors using a retroviral vector. Following each transfection the cells were centrifuged onto a retronectin coated plate and the activated by IL-4, IL-2, and CD40L. Furthermore, we established BiPSCs (BiPSC-A) in which activation-induced cytidine deaminase (AID) could be induced using the doxycycline-controlled. Both the parental BiPSC and BiPSC-A showed the capability of differentiating into hematopoietic progenitor cells (HPCs) based on confirmation of CD34 expression and colony-formation from CD34-positive cells. The findings that BiPSC-A can differentiate into HPCs suggest that there is a possibility that induction of AID expression would result in chromosomal translocations in the process of differentiation from BiPSCs, and therefore that these BiPSCs could be useful in elucidating the tumor origin of abnormal B cells in myelomagenesis. |
| 巻・号 | 7(1) |
| ページ | 1659 |
| 公開日 | 2017-5-10 |
| DOI | 10.1038/s41598-017-01627-1 |
| PII | 10.1038/s41598-017-01627-1 |
| PMID | 28490810 |
| PMC | PMC5431994 |
| MeSH | Animals Antigens, CD19 / metabolism B-Lymphocytes / cytology* B-Lymphocytes / drug effects B-Lymphocytes / metabolism Cell Differentiation* / drug effects Colony-Forming Units Assay Cytidine Deaminase / biosynthesis* Doxycycline / pharmacology Enzyme Induction / drug effects Hematopoiesis / drug effects Hematopoietic Stem Cells / cytology* Hematopoietic Stem Cells / drug effects Hematopoietic Stem Cells / metabolism Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / drug effects Induced Pluripotent Stem Cells / metabolism Kruppel-Like Factor 4 Lymph Nodes / cytology Mice, SCID Models, Biological |
| IF | 3.998 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 10T1/2(RCB0247) HUC-Fm(RCB0197) |