RRC ID 57673
Author Lee YC, Kojima N, Wada E, Kurosawa N, Nakaoka T, Hamamoto T, Tsuji S.
Title Cloning and expression of cDNA for a new type of Gal beta 1,3GalNAc alpha 2,3-sialyltransferase.
Journal J. Biol. Chem.
Abstract Based on the sequences of the highly conserved segments in the previously cloned sialyltransferases, a cDNA encoding a new type of Gal beta 1,3GalNAc alpha 2,3-sialyltransferase (ST3GalA.2) has been isolated from both mouse and rat brain cDNA libraries. The cDNA sequences included an open reading frame coding for 350 amino acids, and the primary structure of this enzyme suggested a putative domain structure consisting of four regions, like that in other glycosyltransferases. The deduced amino acid sequence of ST3GalA.2 (mouse) showed 76% identity in the active domain with that of the previously cloned mouse Gal beta 1,3GalNAc alpha 2,3-sialyltransferase (ST3GalA.1 (Lee, Y.-C., Kurosawa, N., Hamamoto, T., Nakaoka, T., and Tsuji, S. (1993) Eur. J. Biochem. 216, 377-385)). Northern blotting indicated that the expression of ST3GalA.2 mRNA is tissue-specific, it being prominent in brain and liver, while that in the other tissues is very low. This enzyme expressed in COS-7 cells exhibited transferase activity only toward the disaccharide moiety of Gal beta 1,3GalNAc of glycolipids as well as glycoproteins and oligosaccharides like ST3GalA.1, but showed a difference in acceptor substrate preference, i.e. asialo-GM1 and GM1 were much more suitable substrates for ST3GalA.2 than for ST3GalA.1.
Volume 269(13)
Pages 10028-33
Published 1994-4-1
PMID 8144500
MeSH Amino Acid Sequence Animals Base Sequence Brain / enzymology* Cloning, Molecular Conserved Sequence* DNA Primers DNA, Complementary / metabolism Gene Expression* Gene Library Humans Mice Molecular Sequence Data Polymerase Chain Reaction Rats Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Sialyltransferases / biosynthesis* Sialyltransferases / genetics* Sialyltransferases / metabolism Substrate Specificity Swine
IF 4.106
Times Cited 106
Resource
DNA material mST3GalII (RDB02072)