RRC ID 57692
著者 Nakazawa N, Arakawa O, Yanagida M.
タイトル Condensin locates at transcriptional termination sites in mitosis, possibly releasing mitotic transcripts.
ジャーナル Open Biol
Abstract Condensin is an essential component of chromosome dynamics, including mitotic chromosome condensation and segregation, DNA repair, and development. Genome-wide localization of condensin is known to correlate with transcriptional activity. The functional relationship between condensin accumulation and transcription sites remains unclear, however. By constructing the auxin-inducible degron strain of condensin, herein we demonstrate that condensin does not affect transcription itself. Instead, RNA processing at transcriptional termination appears to define condensin accumulation sites during mitosis, in the fission yeast Schizosaccharomyces pombe. Combining the auxin-degron strain with the nda3 β-tubulin cold-sensitive (cs) mutant enabled us to inactivate condensin in mitotically arrested cells, without releasing the cells into anaphase. Transcriptional activation and termination were not affected by condensin's degron-mediated depletion, at heat-shock inducible genes or mitotically activated genes. On the other hand, condensin accumulation sites shifted approximately 500 bp downstream in the auxin-degron of 5'-3' exoribonuclease Dhp1, in which transcripts became aberrantly elongated, suggesting that condensin accumulates at transcriptionally terminated DNA regions. Growth defects in mutant strains of 3'-processing ribonuclease and polyA cleavage factors were additive in condensin temperature-sensitive (ts) mutants. Considering condensin's in vitro activity to form double-stranded DNAs from unwound, single-stranded DNAs or DNA-RNA hybrids, condensin-mediated processing of mitotic transcripts at the 3'-end may be a prerequisite for faithful chromosome segregation.
巻・号 9(10)
ページ 190125
公開日 2019-10-31
DOI 10.1098/rsob.190125
PMID 31615333
PMC PMC6833218
MeSH Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism* Chromosome Segregation DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Exoribonucleases / genetics Exoribonucleases / metabolism Gene Expression Regulation, Fungal Mitosis* Multiprotein Complexes / genetics Multiprotein Complexes / metabolism* Schizosaccharomyces Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Transcription Termination, Genetic* Tubulin / metabolism
IF 4.931
引用数 1
リソース情報
酵母 FY21102