RRC ID 57722
Author Yoshitomi T, Kaminaga S, Sato N, Toyoshima M, Moriyama T, Yoshimoto K.
Title Formation of Spherical Palmelloid Colony with Enhanced Lipid Accumulation by Gel Encapsulation of Chlamydomonas debaryana NIES-2212.
Journal Plant Cell Physiol
Abstract Microalgae such as Chlamydomonas reinhardtii accumulate triacylglycerol (TAG), which is a potential source of biofuels, under stress conditions such as nitrogen deprivation, whereas Chlamydomonas debaryana NIES-2212 has previously been identified and characterized as one of the rare species of Chlamydomonas, which massively accumulates TAG in the stationary phase without external stress. As the high density of the cells in the stationary phase was supposed to act as a trigger for the accumulation of TAG in C. debaryana, in this study, C. debaryana was encapsulated in a Ca2+-alginate gel for the culture with high cell density. We discovered that the growth of the encapsulated cells resulted in the formation of spherical palmelloid colonies with high cell density, where daughter cells with truncated flagella remained wrapped within the mother cell walls. Interestingly, gel encapsulation markedly promoted proliferation of C. debaryana cells, and the encapsulated cells reached the stationary phase earlier than that of the free-living cells. Gel encapsulation also enhanced TAG accumulation. Gene expression analysis revealed that two genes of acyltransferases, DGAT1 and DGTT3, were upregulated in the stationary phase of free-living C. debaryana. In addition, the gene expression of these acyltransferases increased earlier in the encapsulated cells than that in the free-living cells. The enhanced production of TAG by alginate gel encapsulation was not found in C. reinhardtii which is known to use a different repertoire of acyltransferases in lipid accumulation.
Volume 61(1)
Pages 158-168
Published 2020-1-1
DOI 10.1093/pcp/pcz188
PII 5582679
PMID 31589321
MeSH Acyltransferases / genetics Acyltransferases / metabolism Alginates Biofuels Cell Proliferation Chlamydomonas / cytology Chlamydomonas / genetics Chlamydomonas / growth & development* Chlamydomonas / metabolism* Chlorophyll / analysis Diacylglycerol O-Acyltransferase / genetics Diacylglycerol O-Acyltransferase / metabolism Fatty Acids / metabolism Gels Gene Expression Regulation, Plant Microalgae / metabolism* Nitrogen / metabolism Plant Proteins / genetics Plant Proteins / metabolism Transcriptome Triglycerides / metabolism*
IF 4.062
Times Cited 1
Algae NIES-2212